Anti-CASK 抗体 (ab3383)
Key features and details
- Rabbit polyclonal to CASK
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-CASK antibody
CASK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CASK -
由来種
Rabbit -
特異性
By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human
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免疫原
Synthetic peptide corresponding to Human CASK aa 578-592.
Sequence:CERDSPSTSRQSPAN
(Peptide available asab4961) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. |
ターゲット情報
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機能
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. -
組織特異性
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver. -
関連疾患
Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus.
Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation. -
配列類似性
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
ドメイン
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2. -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8573 Human
- Entrez Gene: 12361 Mouse
- Entrez Gene: 29647 Rat
- Omim: 300172 Human
- SwissProt: O14936 Human
- SwissProt: O70589 Mouse
- SwissProt: Q62915 Rat
- Unigene: 495984 Human
see all -
別名
- CAGH39 antibody
- Caki antibody
- Calcium/calmodulin dependent serine protein kinase antibody
see all
画像
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Western blot - Anti-CASK antibody (ab3383)Western blot detection of CASK on AtT20 cell lysate using ab3383.
プロトコール
データシートおよび資料
参考文献 (2)
ab3383 は 2 報の論文で使用されています。
- Xiao X et al. N-wasp is required for structural integrity of the blood-testis barrier. PLoS Genet 10:e1004447 (2014). PubMed: 24967734
- Ojeh N et al. The MAGUK-family protein CASK is targeted to nuclei of the basal epidermis and controls keratinocyte proliferation. J Cell Sci 121:2705-17 (2008). IHC ; Mouse, Rat . PubMed: 18664494
