Key features and details
- Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品名Anti-Casein Kinase 2 beta (phospho S209) antibody
Casein Kinase 2 beta 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
特異性Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2].
アプリケーション適用あり: WBmore details
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- HeLa cell lysates or K562 cell lysates.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209.
The Abpromise guarantee
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
配列類似性Belongs to the casein kinase 2 subunit beta family.
翻訳後修飾Phosphorylated by alpha subunit.
- Information by UniProt
- Casein kinase 2 beta polypeptide antibody
- Casein kinase II beta subunit antibody
- Casein kinase II subunit beta antibody
Peptide Competition and Phosphatase TreatmentLysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with ab12861 antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method.
The data show that only the peptide corresponding to ab12861 blocks the signal, verifying the specificity of the antibody.
Peptide Competition and Phosphatase Treatment