リコンビナントRabMAb

Anti-Casein Kinase 1 gamma 2 抗体 [EPR20918-13] - BSA and Azide free (ab270040)

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Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
  • Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
  • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
  • Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20918-13] to Casein Kinase 1 gamma 2 - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free
    Casein Kinase 1 gamma 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20918-13] to Casein Kinase 1 gamma 2 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Mouse
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T transfected with human Casein Kinase 1 gamma 2; HEK-293T transfected with mouse Casein Kinase 1 gamma 2; Mouse and rat testis lysate. Flow Cyt: MEF and HEK-293T cells. IP: Mouse testis lysate.

  • 特記事項

    ab270040 is the carrier-free version of ab238121. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IP
Mouse
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
特記事項
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
  • 追加情報
    Is unsuitable for ICC/IF,IHC-Fr or IHC-P.

    • ターゲット情報

    • 機能

      Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.

    • 組織特異性

      Testis.

    • 配列類似性

      Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Autophosphorylated.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Casein kinase 1, gamma 2 antibody
        • Casein kinase I isoform gamma 2 antibody
        • Casein kinase I isoform gamma-2 antibody
        • CK1g2 antibody
        • CKI gamma 2 antibody
        • CKI-gamma 2 antibody
        • CSNK1G 2 antibody
        • Csnk1g2 antibody
        • KC1G2_HUMAN antibody
        see all

      画像

      • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
        Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

        Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238121).

      • Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
        Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

        Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

        Lane 1: Mouse testis tissue lysate 10 ug

        Lane 2: ab238121 IP in mouse testis tissue lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238121 in mouse testis tissue lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 32 seconds.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238121).

      • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
        Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

        Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct ) labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

        A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238121).

      • Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
        Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270040 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270040 は論文での使用が確認できていません。

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