Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7121] to CARS
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
製品名Anti-CARS antibody [EPR7121]
CARS 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7121] to CARS
アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
A synthetic peptide corresponding to residues in human CARS (P49589)
- 293T cell lysate HeLa whole cell lysate (ab150035) U87-MG cell lysate BxPC-3 cell lysate MCF-7 cell lysate human breast carcinoma tissue A549 cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（KD 値）KD = 5.70 x 10 -11 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab126714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 85 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval step is recommended.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
関連疾患Note=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
配列類似性Belongs to the class-I aminoacyl-tRNA synthetase family.
- Information by UniProt
- Cysteine tRNA ligase antibody
- Cars antibody
- CysRS antibody
All lanes : Anti-CARS antibody [EPR7121] (ab126714) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : BxPC-3 cell lysate
Lane 5 : MCF-7 cell lysate
Lane 6 : A549 cell lysate
Predicted band size: 85 kDa
ab126714, at 1/50 dilution, stains CARS in paraffin-embedded human breast carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD