Anti-Cardiac Troponin T 抗体 [1F11] (ab10214)
Key features and details
- Mouse monoclonal [1F11] to Cardiac Troponin T
- Suitable for: Sandwich ELISA, ICC
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-Cardiac Troponin T antibody [1F11]
Cardiac Troponin T 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1F11] to Cardiac Troponin T -
由来種
Mouse -
特異性
Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813) -
アプリケーション
適用あり: Sandwich ELISA, ICCmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Tissue, cells or virus corresponding to Human Cardiac Troponin T. Free human cardiac troponin T
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エピトープ
Peptide 146-160. -
ポジティブ・コントロール
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)
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特記事項
This product was changed from ascites to tissue culture supernatant on 30th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Concentration varies from lot to lot and can be provided on request.
Does not cross-react with skeletal muscle troponin T, cTnI or TnC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purity tested by electrophoresis. -
一次抗体 備考
Does not cross-react with skeletal muscle troponin T, cTnI or TnC. -
ポリ/モノ
モノクローナル -
クローン名
1F11 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10214の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
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ICC | (2) |
Use a concentration of 1 - 5 µg/ml.
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特記事項 |
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection. |
ICC
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
組織特異性
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
関連疾患
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
配列類似性
Belongs to the troponin T family. - Information by UniProt
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参照データベース
- Entrez Gene: 7139 Human
- Omim: 191045 Human
- SwissProt: P45379 Human
- Unigene: 533613 Human
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別名
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
画像
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Immunofluorescence staining of Cardiac Troponin T using ab10214 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab10214 at 1 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab10214 also gave a positive staining using 4% formaldehyde fixation (10 min).
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Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5µg/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5µg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (59)
ab10214 は 59 報の論文で使用されています。
- Haryono A et al. ChGn-2 Plays a Cardioprotective Role in Heart Failure Caused by Acute Pressure Overload. J Am Heart Assoc 11:e023401 (2022). PubMed: 35322673
- Li X et al. Oxidative Stress Contributes to Cytoskeletal Protein Degradation of Esox lucius through Activation of Mitochondrial Apoptosis during Postmortem Storage. Foods 11:N/A (2022). PubMed: 35564031
- He Y et al. A smart adhesive Janus hydrogel for non-invasive cardiac repair and tissue adhesion prevention. Nat Commun 13:7666 (2022). PubMed: 36509756
- Wang Q et al. Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells. Sci China Life Sci 64:269-281 (2021). PubMed: 32712833
- Pierre M et al. NaV1.5 knockout in iPSCs: a novel approach to study NaV1.5 variants in a human cardiomyocyte environment. Sci Rep 11:17168 (2021). PubMed: 34433864