Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Rat Tissue lysate - other (Left Ventrical, Heart)
Specification
Left Ventrical, Heart
Total protein in input
500 µg
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pro2 buffer
Dilution
1/50
Western Blot antibody
Name
Non-Abcam antibody was used: Troponin I
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Additional data
Additional Notes
Sham Rat heart LV tissue (n=4) was homogenized in Pro2 buffer to maintain protein-protein interactions. From the same animal, whole LV tissue, IP 'soup', and Troponin T (TnT) co-IP samples were run on the same gel. The gel was transferred to PVDF membrane and probed for Troponin I (TnI). Feel free to contact me if you would like further information.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Aaron West
Verified customer
投稿 Feb 18 2014