Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Tx100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum 10% BSA 0.1% Tx100 PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
Additional data
PubMed ID
21654746: View
Additional Notes
Fixation: 4% paraformaldehyde, 90 mins (whole heart)
Permeabilisation: 0.5% Tx100 in PBS
Block?Ab incubation: 1% goat serum 10% BSA 0.1% Tx100 PBS
Permeabilisation: 0.5% Tx100 in PBS
Block?Ab incubation: 1% goat serum 10% BSA 0.1% Tx100 PBS
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 18 2012