Anti-Cardiac Troponin T 抗体 [1C11] (ab8295)

Reviews (9)Q&A (12)References (127)

Key features and details

  • Mouse monoclonal [1C11] to Cardiac Troponin T
  • Suitable for: IHC-P, Sandwich ELISA
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cardiac Troponin T antibody [1C11]
    Cardiac Troponin T 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1C11] to Cardiac Troponin T

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Sandwich ELISAmore details

  • 種交差性

    交差種: Mouse, Rat, Dog, Human

  • 免疫原

    Other Immunogen Type corresponding to Human Cardiac Troponin T aa 171-190.
    Database link: P45379

  • ポジティブ･コントロール

    • Natural Human Cardiac Troponin T protein (ab9937) can be used as a positive control in WB. IHC: Human heart tissue. ICC/IF: Cardiomyocytes.

  • 特記事項

    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
AP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration. Can be used as Capture or Detection antibody.

ターゲット情報

  • 機能

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

  • 組織特異性

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

  • 関連疾患

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.

  • 配列類似性

    Belongs to the troponin T family.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Ye L et al. Decreased Yes-Associated Protein-1 (YAP1) Expression in Pediatric Hearts with Ventricular Septal Defects. PLoS One 10:e0139712 (2015).

    Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).

  • Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
    Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
    Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 µg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
    Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

参考文献 (127)

ab8295 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8295 は 127 報の論文で使用されています。

  • Wan Z  et al. Mononuclear phagocyte system blockade improves therapeutic exosome delivery to the myocardium. Theranostics 10:218-230 (2020). PubMed: 31903116
  • Kim AR  et al. Screening ginseng saponins in progenitor cells identifies 20(R)-ginsenoside Rh2 as an enhancer of skeletal and cardiac muscle regeneration. Sci Rep 10:4967 (2020). PubMed: 32188912
  • Rao P  et al. Near-infrared light driven tissue-penetrating cardiac optogenetics via upconversion nanoparticles in vivo. Biomed Opt Express 11:1401-1416 (2020). PubMed: 32206418
  • Williams JL  et al. Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not. Life Sci Alliance 3:N/A (2020). PubMed: 32213617
  • Wong AO  et al. Combinatorial Treatment of Human Cardiac Engineered Tissues With Biomimetic Cues Induces Functional Maturation as Revealed by Optical Mapping of Action Potentials and Calcium Transients. Front Physiol 11:165 (2020). PubMed: 32226389
View all Publications for this product

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1-10 of 21 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1C11]

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Heart)
Permeabilization
Yes - TritonX
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 13 2020

Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1C11]

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Cardiomyocyte)
Permeabilization
Yes - TritonX
Specification
Cardiomyocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 06 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cardiac)
Permeabilization
Yes - Triton X
Specification
Cardiac
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Feb 05 2020

Flow Cytometry abreview for Anti-Cardiac Troponin T antibody [1C11]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (Human embryonic stem cell derived cardiomyocytes)
Specification
Human embryonic stem cell derived cardiomyocytes
Permeabilization
Yes - Triton
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Abcam user community

Verified customer

投稿 Dec 16 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Nov 19 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Sample
Human Cell (iPS cell derived cardiomyocyte)
Specification
iPS cell derived cardiomyocyte
Permeabilization
Yes - 0.2% triton X
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Oct 14 2014

Immunoprecipitation abreview for Anti-Cardiac Troponin T antibody [1C11]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Rat Tissue lysate - other (Left Ventrical, Heart)
Specification
Left Ventrical, Heart
Total protein in input
500 µg
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Aaron West

Verified customer

投稿 Feb 18 2014

Question

Good morning,

I would like to know the isoelectric point (pI) of the product Anti-Cardiac Troponin T antibody [1C11] (ab8295).

Thank you.

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Answer

Thank you for your e-mail.

Unfortunately we have not determined the pI for ab8295:Anti-Cardiac Troponin T antibody. I am sorry for the inconvenience.

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

I have a technical enquiry for the 2 products below:



∙ Anti-Cardiac Troponin T antibody [1C11] (Biotin), ref ab105441 from Abcam (https://www.abcam.com/Cardiac-Troponin-T-antibody-1C11-Biotin-ab105441.html)



∙ Cardiac Troponin T protein, ref ab9937 from Abcam (https://www.abcam.com/Cardiac-Troponin-T-protein-ab9937.html)



As far as I have understood, the cardiac Troponin T protein mentioned above (ab9937) is recommended for use with two different antibodies (ab8295 & ab8296). Can you please confirm that the ab105441 is the biotinilated version of ab8295 and that its affinity with the cardiac troponin T (ab9937) will be adequate.



Looking forward for your response.

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Answer

Thank you for contacting us.

Yes, I can confirm the ab105441is the biotinylated form of ab8295, and this antibody shows a good affinity towards the native protein as can be seen in the ELISA and IHC data.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Question

cardiac troponin ELISA antibodies with protein for troponin T , I or C

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Answer

Thank you very much for your patience while I was investigating.


I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Troponin T,I have also double checked with the source of the antibodies, and they do have a protein which they can recommend. I am currently checking with my colleagues if we can add it to our catalog. However we do have the individual standard proteins or also a recomplexed protein, which could be tested also as a standard. Please let me know what you think. Indeed, it could be that the recomplexed protein is more stable and less prone to protein degradation and you could use it for all the three ELISAs. potential standard complex: ab94712 https://www.abcam.com/index.html?datasheet=94712 (or use the following: https://www.abcam.com/index.html?datasheet=94712).

Troponin C:
capture antibody: ab8285 https://www.abcam.com/index.html?datasheet=8285 (or use the following: https://www.abcam.com/index.html?datasheet=8285).
detection antibody: ab8286 https://www.abcam.com/index.html?datasheet=8286 (or use the following: https://www.abcam.com/index.html?datasheet=8286).
potential standard protein: ab68911, ab70106 or ab9932 https://www.abcam.com/index.html?datasheet=68911 (or use the following: https://www.abcam.com/index.html?datasheet=68911).

Troponin I:
capture antibody: ab115704 https://www.abcam.com/index.html?datasheet=115704 (or use the following: https://www.abcam.com/index.html?datasheet=115704).
detection antibody: ab19615 https://www.abcam.com/index.html?datasheet=19615 (or use the following: https://www.abcam.com/index.html?datasheet=19615).
potential standard protein: ab50822 https://www.abcam.com/index.html?datasheet=50822 (or use the following: https://www.abcam.com/index.html?datasheet=50822).

Troponin T:
capture antibody: ab9937 https://www.abcam.com/index.html?datasheet=9937 (or use the following: https://www.abcam.com/index.html?datasheet=9937).
detection antibody: ab19615 https://www.abcam.com/index.html?datasheet=19615 (or use the following: https://www.abcam.com/index.html?datasheet=19615).
potential standard protein: ab9937, evt new one https://www.abcam.com/index.html?datasheet=9937 (or use the following: https://www.abcam.com/index.html?datasheet=9937).

For all the proteins or for the complex, we are happy to provide you with our testing discount code as well. Please let me know whether you are interested and I am happy to send you the detailed description of this offer (you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product - for more information, please see also https://www.abcam.com/test-vial)

Unfortunately we do not have any ELISA kits for the moment against the cardiac Troponins. We are however looking into adding them to our catalog within a few months.

I hope this information is helpful. Please do not hesitate to contact me again, should you have any additional questions or should you like to receive the testing discount.

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1-10 of 21 Abreviews or Q&A

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