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  1. Link

    cardiac-troponin-t-antibody-1c11-ab8295.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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Anti-Cardiac Troponin T 抗体 [1C11] (ab8295)

  • Datasheet
  • SDS
Reviews (12)Q&A (12)References (187)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
  • Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
  • Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)

Key features and details

  • Mouse monoclonal [1C11] to Cardiac Troponin T
  • Suitable for: ICC, IHC-P, Sandwich ELISA
  • Reacts with: Mouse, Dog, Human
  • Isotype: IgG1

こちらの製品もご検討ください

細胞
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ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)
タンパク質
Product image
Recombinant Human Cardiac Troponin T protein (ab86685)
一次抗体
Product image
Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

関連製品

製品の概要

  • 製品名

    Anti-Cardiac Troponin T antibody [1C11]
    Cardiac Troponin T 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [1C11] to Cardiac Troponin T
  • 由来種

    Mouse
  • アプリケーション

    適用あり: ICC, IHC-P, Sandwich ELISAmore details
  • 種交差性

    交差種: Mouse, Dog, Human
  • 免疫原

    Other Immunogen Type corresponding to Human Cardiac Troponin T aa 171-190.
    Database link: P45379

  • ポジティブ・コントロール

    • Natural Human Cardiac Troponin T protein (ab9937) can be used as a positive control in WB. IHC: Human heart tissue. ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612).
  • 特記事項

    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.

    This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    Purified from TCS
  • 一次抗体 備考

    This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.
  • ポリ/モノ

    モノクローナル
  • クローン名

    1C11
  • ミエローマ

    Sp2/0
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin
    • Cardiovascular
    • Cardiovascular Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes
    • Signal Transduction
    • Antibodies
    • cardiac troponin t

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Cardiac Troponin T protein (ab86685)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
    • ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8295の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (2)
Use a concentration of 1 - 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use at an assay dependent concentration. Can be used as Capture or Detection antibody.
特記事項
ICC
Use a concentration of 1 - 5 µg/ml.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use at an assay dependent concentration. Can be used as Capture or Detection antibody.

ターゲット情報

  • 機能

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 組織特異性

    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 関連疾患

    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 配列類似性

    Belongs to the troponin T family.
  • Target information above from: UniProt accession P45379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 403532 Dog
    • Entrez Gene: 7139 Human
    • Entrez Gene: 21956 Mouse
    • Omim: 191045 Human
    • SwissProt: P45379 Human
    • SwissProt: P50752 Mouse
    • Unigene: 533613 Human
    • Unigene: 247470 Mouse
    • 別名

      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Ye L et al. Decreased Yes-Associated Protein-1 (YAP1) Expression in Pediatric Hearts with Ventricular Septal Defects. PLoS One 10:e0139712 (2015).

      Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).

    • Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
      Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)

      Immunofluorescence staining of Cardiac Troponin T using ab8295 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.


      The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8295 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


      The antibody ab8295 gave comparable results using MeOH fixation (100%, 5 min).

    • Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
      Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
      Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1 µg/well, detection ab1454 [clone 7E7], 200 ng/well. Assay time, 30 min at room temperature.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)
      Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (187)

    ab8295 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8295 は 187 報の論文で使用されています。

    • Gladka MM  et al. Cardiomyocytes stimulate angiogenesis after ischemic injury in a ZEB2-dependent manner. Nat Commun 12:84 (2021). PubMed: 33398012
    • Jiang Y  et al. Myocardial repair of bioengineered cardiac patches with decellularized placental scaffold and human-induced pluripotent stem cells in a rat model of myocardial infarction. Stem Cell Res Ther 12:13 (2021). PubMed: 33413626
    • Liang D  et al. Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nat Commun 12:287 (2021). PubMed: 33436583
    • Takashima S  et al. Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells. Sci Rep 11:1520 (2021). PubMed: 33452355
    • Molenaar B  et al. Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair. Commun Biol 4:146 (2021). PubMed: 33514846
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 24 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 16 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Human vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 16 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cardiomyocyte)
    Permeabilization
    Yes - TritonX
    Specification
    Cardiomyocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 26 2021

    Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1C11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Heart)
    Permeabilization
    Yes - TritonX
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 13 2020

    Immunocytochemistry abreview for Anti-Cardiac Troponin T antibody [1C11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Cardiomyocyte)
    Permeabilization
    Yes - TritonX
    Specification
    Cardiomyocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Cardiac)
    Permeabilization
    Yes - Triton X
    Specification
    Cardiac
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 05 2020

    Flow Cytometry abreview for Anti-Cardiac Troponin T antibody [1C11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Sample
    Human Cell (Human embryonic stem cell derived cardiomyocytes)
    Specification
    Human embryonic stem cell derived cardiomyocytes
    Permeabilization
    Yes - Triton
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 16 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Specification
    Human pluripotent stem cell derived cardiomyocyte
    Permeabilization
    Yes - saponin
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 19 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin T antibody [1C11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Sample
    Human Cell (iPS cell derived cardiomyocyte)
    Specification
    iPS cell derived cardiomyocyte
    Permeabilization
    Yes - 0.2% triton X
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 14 2014

    Immunoprecipitation abreview for Anti-Cardiac Troponin T antibody [1C11]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Rat Tissue lysate - other (Left Ventrical, Heart)
    Specification
    Left Ventrical, Heart
    Total protein in input
    500 µg
    Read More

    Aaron West

    Verified customer

    投稿 Feb 18 2014

    1-10 of 24 Abreviews or Q&A

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