製品の概要

  • 製品名

    Anti-Cardiac Troponin I antibody
    Cardiac Troponin I 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Cardiac Troponin I
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICC/IF, IHC-Fr, IHC-P, Flow Cyt, ELISAmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human Cardiac Troponin I aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab47002)

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following tissue lysates: Heart (human) tissue lysate, mouse heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Cardiac Troponin I peptide (ab47002).
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/100.
IHC-P 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 21909276

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 関連疾患

    Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性

    Belongs to the troponin I family.
  • Information by UniProt
  • 参照データベース

  • 別名

    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

画像

  • All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

  • IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab74003 staining Cardiac Troponin I in neonatal Rat cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400 in 3% BSA + PBS-T) for 12 hours at 4°C. An AlexaFluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?
    Additional bands at: 160 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution

    All lanes : Pig Heart whole cell lysate

    Lysates/proteins at 4 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 29 kDa why is the actual band size different from the predicted?



    Blocking Step: 20% Serum for 3 hours at 24°C

    See Abreview

  • Immunohistochemical analysis of frozen mouse heart tissue labeling Cardiac Troponin I with ab47003 at 1/100 dilution.

    See Abreview

  • ab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:

  • Onwuli DO  et al. The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. Int J Cardiol N/A:N/A (2019). Read more (PubMed: 30772011) »
  • Suwa Y  et al. Old-Age Onset Progressive Cardiac Contractile Dysfunction in a Patient with Polycystic Kidney Disease Harboring a PKD1 Frameshift Mutation. Int Heart J 60:220-225 (2019). Read more (PubMed: 30464138) »
See all 65 Publications for this product

レビューと Q&A

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1-10 of 16 Abreviews

Application
ELISA
Sample
Human Serum (immobilization on beads)
Specification
immobilization on beads
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Type
Sandwich (Capture)

Mr. Chang Su Jeon

Verified customer

投稿 Sep 19 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Mrs. talya razin

Verified customer

投稿 Jun 11 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (post MI mouse heart)
Permeabilization
Yes - 3% triton
Specification
post MI mouse heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 May 29 2019

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cultured Cells (Fetal Cardiomyocytes)
Specification
Fetal Cardiomyocytes
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde

Jennifer Dewing

Verified customer

投稿 Mar 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Nov 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 26 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 22 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult heart)
Specification
Adult heart
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Tx100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jun 18 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neonatal cardiomyocyte)
Specification
neonatal cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Mrs. Jamie Soto

Verified customer

投稿 Jun 15 2012

Application
Western blot
Sample
Human Tissue lysate - whole (heart)
Loading amount
10 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jun 07 2012

1-10 of 16 Abreviews

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