Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cardiomyocyte (heart cell))
Permeabilization
Yes - 0.1 % Triton X
Specification
Cardiomyocyte (heart cell)
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: goat serum
Concentration
10 µg/ml
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa 488 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Neerajha Nagarajan
Verified customer
投稿 Mar 14 2017