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Anti-Carbonic Anhydrase 9/CA9 抗体 [2D3] (ab107257)

Reviews (2)Q&A (3)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
  • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
  • Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)

Key features and details

  • Mouse monoclonal [2D3] to Carbonic Anhydrase 9/CA9
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Carbonic Anhydrase 9/CA9 antibody [2D3]
    Carbonic Anhydrase 9/CA9 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2D3] to Carbonic Anhydrase 9/CA9

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human Carbonic Anhydrase 9/CA9.
    Database link: Q16790

  • ポジティブ･コントロール

    • Hela and A549 cell lysate; lung and colonic tissues and NTERA-2 cells.

  • 特記事項

    This product was changed from ascites to supernatant. Lot no’s high than GR311638-1 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab107257の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (1)
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
IHC-P (1)
1/200 - 1/1000.
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
IHC-P
1/200 - 1/1000.

ターゲット情報

  • 機能

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

  • 組織特異性

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

  • 配列類似性

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.

  • 翻訳後修飾

    Asn-346 bears high-mannose type glycan structures.

  • 細胞内局在

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CA-IX antibody
      • CA9 antibody
      • CAH9_HUMAN antibody
      • CAIX antibody
      • Carbonate dehydratase IX antibody
      • Carbonic anhydrase 9 antibody
      • Carbonic anhydrase IX antibody
      • Carbonic dehydratase antibody
      • G250 antibody
      • Membrane antigen MN antibody
      • MN antibody
      • P54/58N antibody
      • pMW1 antibody
      • RCC associated protein G250 antibody
      • RCC-associated antigen G250 antibody
      • Renal cell carcinoma-associated antigen G250 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
      Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using 1/200 ab107257 with DAB staining.
    • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
      Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
      All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257) at 1/500 dilution

      Lane 1 : Hela Cell lysate
      Lane 2 : A549 Cell Lysate

      Predicted band size: 50 kDa

    • Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)
      Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257)

      Flow cytometric analysis of NTERA-2 cells using Carbonic Anhydrase 9/CA9 mouse ab107257 in green and negative control in purple.

    参考文献 (8)

    ab107257 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab107257 は 8 報の論文で使用されています。

    • Mishra CB  et al. Anti-breast cancer action of carbonic anhydrase IX inhibitor 4-[4-(4-Benzo[1,3]dioxol-5-ylmethyl-piperazin-1-yl)-benzylidene-hydrazinocarbonyl]-benzenesulfonamide (BSM-0004): in vitro and in vivo studies. J Enzyme Inhib Med Chem 36:954-963 (2021). PubMed: 33947294
    • Chen W  et al. Identification of anaplastic lymphoma kinase fusions in clear cell renal cell carcinoma. Oncol Rep 43:817-826 (2020). PubMed: 32020234
    • Suri BK  et al. Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1a,25-dihydroxyvitamin D3. Exp Dermatol 28:976-980 (2019). PubMed: 31125456
    • Brahmer A  et al. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation. J Extracell Vesicles 8:1615820 (2019). PubMed: 31191831
    • Yang WJ  et al. Overexpression of Tie2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 15:8027-8033 (2018). PubMed: 29849805
    • Sun W  et al. Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression. Sci Rep 7:7190 (2017). PubMed: 28775317
    • Amiri A  et al. Inhibition of carbonic anhydrase IX in glioblastoma multiforme. Eur J Pharm Biopharm 109:81-92 (2016). PubMed: 27702686
    • Schweiger T  et al. Carbonic anhydrase IX is associated with early pulmonary spreading of primary colorectal carcinoma and tobacco smoking. Eur J Cardiothorac Surg N/A:N/A (2013). Human . PubMed: 24321995

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Carbonic Anhydrase IX antibody [2D3]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Gastric cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    Yes - Triton 0.025%
    Specification
    Gastric cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 03 2015

    Western blot abreview for Anti-Carbonic Anhydrase IX antibody [2D3]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma primary cells)
    Loading amount
    50 µg
    Specification
    Glioblastoma primary cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 21 2012

    Question

    Product code: 107257
    Lot number: GR68366-1
    Inquiry: I recieved this antibody yesterday in a tube that was supposed to contain 100uL. When I aliquoted the antibody to freeze (as per your storage instructions) I found there to only be 70uL in the tube, despite spinning the tube to ensure I extracted all of its contents. Obviously given the expense off this product I am very disapointed to find nearly 1/3rd of the expected amout missing. My order ref is 1109937. Please advise.

    Read More
    Answer

    Thank you for contacting us.
    I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of 1112020.
    Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    I was wondering if you offer a sample size for your carbonic anhydrase IX monoclonal antibody? I was hoping to test out the antibody prior to purchasing it.

    Read More
    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Question

    Hello, I am writing you re your Indirect ELISA kits and hope you can advise which kit buy and protocol to use. We have developed RCC mouse model using  RENCA mouse RCC cells over expressing the human CAIX protein. We would like to test/control for the possible presence of mouse anti human CAIX antibodies in the mouse sera. That is to say we would like to coat the plates with recombinant human CAIX protein, use OUR mice sera as primary Abs (as a “detector Antibodies”) and then follow with a secondary Anti Mouse Ab  HRP Conjugated/other detection conjugate. Could you possibly advise which kit buy and protocol to use. Thank you

    Read More
    Answer

    Thank you for contacting us. Unfortunately we do not have any kit corresponding to the detection of the CAIX protein. However, I would suggest to develop an ELISA assay using the CAIX protein ab114200 that would be coated on the plate. A control anti-CAIX, such as ab15086, for which the concentration is known, may be used to create a standard curve and to quantify the amount of anti-CAIX antibodies contained in the mouse sera. ab15086 is a rabbit antibody. A mouse antibody would ideal, unfortunately, the only mouse anti-CAIX we have (ab107257) is not purified. It is an ascites surpernatant and the concentration has therefore not been determined. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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