リコンビナントRabMAb

Anti-Carbonic Anhydrase 1/CA1 抗体 [EPR23232-286] - BSA and Azide free (ab269951)

Submit a review Submit a question
Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
  • Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23232-286] to Carbonic Anhydrase 1/CA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free
    Carbonic Anhydrase 1/CA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23232-286] to Carbonic Anhydrase 1/CA1 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEL, TF-1, Human lung, Human pancreas, Human colon, Mouse spleen, Mouse colon and Rat spleen lysates. IHC-P: Human bone marrow, Human lung, Human colon, Mouse colon and Rat colon tissues. Flow Cyt: HEL cells.

  • 特記事項

    ab269951 is the carrier-free version of ab267475. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23232-286

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC/IF or IP.

    • ターゲット情報

    画像

    • Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEL (Human Erythroleukemia erythroblast) cells labelling Carbonic Anhydrase 1/CA with ab267475 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267475).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

      Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat colon (PMID: 21763241). The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267475).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

      Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse colon (PMID: 21763241). The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267475).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 21763241). The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267475).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red blood cells in human lung is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267475).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

      Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human bone marrow is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267475).

    • Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)
      Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] - BSA and Azide free (ab269951)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269951 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269951 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab269951.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com