Application
Immunohistochemistry free floating
Sample
Mouse Cell (Brain)
Specification
Brain
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer (10%NGS in 1XPBS)
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: AF-488 conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
4% paraformaldehyde for mouse intracardial perfusion (5 min) and fixation of mouse brain (overnight), cryoprotected in 30% sucrose in 1XPBS for months. Cryostat sectioning (50µm in thickness), free-floating immunofluorescent staining.
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投稿 Jan 25 2022