Anti-Cannabinoid Receptor II 抗体 (ab3561)
Key features and details
- Rabbit polyclonal to Cannabinoid Receptor II
- Suitable for: ICC, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cannabinoid Receptor II antibody
Cannabinoid Receptor II 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cannabinoid Receptor II -
由来種
Rabbit -
特異性
We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.
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アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).
Database link: Q9QZN9 -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC | Use at an assay dependent concentration. | |
WB | 1/50 - 1/500. Predicted molecular weight: 40 kDa. | |
IHC-P | 1/10 - 1/100. |
ターゲット情報
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機能
Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. -
組織特異性
Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level). -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor. -
細胞内局在
Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons. - Information by UniProt
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参照データベース
- Entrez Gene: 1269 Human
- Entrez Gene: 57302 Rat
- Omim: 605051 Human
- SwissProt: P34972 Human
- SwissProt: Q9QZN9 Rat
- Unigene: 73037 Human
- Unigene: 48776 Rat
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別名
- Cannabinoid receptor 2 antibody
- Cannabinoid receptor 2 macrophage antibody
- CB 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution
Lane 1 : HT29 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Rat colon cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.
プロトコール
参考文献 (53)
ab3561 は 53 報の論文で使用されています。
- Remiszewski P et al. Chronic Cannabidiol Administration Fails to Diminish Blood Pressure in Rats with Primary and Secondary Hypertension Despite Its Effects on Cardiac and Plasma Endocannabinoid System, Oxidative Stress and Lipid Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32075117
- Gupta B et al. Anti-apoptotic and Immunomodulatory Effect of CB2 Agonist, JWH133, in a Neonatal Rat Model of Hypoxic-Ischemic Encephalopathy. Front Pediatr 8:65 (2020). PubMed: 32175293
- Huang W et al. NOX2 is involved in CB2-mediated protection against lung ischemia-reperfusion injury in mice. Int J Clin Exp Pathol 13:277-285 (2020). PubMed: 32211110
- Jing N et al. Exogenous activation of cannabinoid-2 receptor modulates TLR4/MMP9 expression in a spinal cord ischemia reperfusion rat model. J Neuroinflammation 17:101 (2020). PubMed: 32248810
- Baranowska-Kuczko M et al. Vasodilatory effects of cannabidiol in human pulmonary and rat small mesenteric arteries: modification by hypertension and the potential pharmacological opportunities. J Hypertens 38:896-911 (2020). PubMed: 31800399