Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic stelate cells)
Specification
pancreatic stelate cells
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH=6
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/50
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Vector Lab Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Additional Notes
Cannabinoid Receptor I is expressed by pancreatic stellate cells as expected.
Cannabinoids Reduce Markers of Inflammation and Fibrosis in Pancreatic Stellate Cells by Christoph W. Michalski et al, 2008, PlosOne
Cannabinoids Reduce Markers of Inflammation and Fibrosis in Pancreatic Stellate Cells by Christoph W. Michalski et al, 2008, PlosOne
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 28 2011