製品の概要
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製品名Anti-Cannabinoid Receptor I antibody
Cannabinoid Receptor I 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Cannabinoid Receptor I
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由来種Rabbit
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アプリケーション適用あり: WB, IHC-P, IHC-Fr, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Dog, Human
交差が予測される動物種: Cow, Chimpanzee, Macaque monkey
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免疫原
Synthetic peptide:
MSVSTDTSAE AL
, corresponding to C terminal amino acids 461-472 of Human Cannabinoid Receptor I(Peptide available as ab50542.) -
ポジティブ･コントロール
- Human cerebellum or murine brain sections.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab23703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).Can be blocked with Cannabinoid Receptor I peptide (ab50542). | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/300. Detection with an ABC system is recommended. | |
| ICC/IF | 1/50. |
ターゲット情報
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機能Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
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組織特異性Widely expressed.
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配列類似性Belongs to the G-protein coupled receptor 1 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 100299449 Cow
- Entrez Gene: 481913 Dog
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: Q5IS73 Chimpanzee
- SwissProt: P21554 Human
see all -
別名
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
画像
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Western blot - Anti-Cannabinoid Receptor I antibody (ab23703)Anti-Cannabinoid Receptor I antibody (ab23703) + Human cerebellum at 30 µg
Predicted band size: 53 kDa
Observed band size: 60 kDa (why is the actual band size different from the predicted?)Secondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Immunoperoxidase staining of CA region of rat hippocampus with the Cannabinoid Receptor I antibody (ab23703, 4ug/ml). the majority of the protein is localized in the pre-synaptic axons, shown as brown particulaes. A few neurons also show cytoplasm staining. -
Immunohistochemistry (Frozen sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Image from Laurén HB et al, PLoS One. 2010 May 20;5(5):e10733, Fig 5.ab23703 staining Cannabinoid Receptor I in rat brain tissue by Immunohistochemistry (Frozen sections). After fixation in 4% PFA in PBS (pH 7.4), brains were cryoprotected in 30% sucrose in PBS at +4°C, frozen, and thereafter kept at -80°C until used. For the immunostaining, brains were cryosectioned in 30 µm slices, collected in PBS (pH 7.4), and processed in a free-floating system. Slices were first incubated in the blocking solution (BS) containing 2% bovine serum albumin, 2% goat serum, and 0.1% Triton X-100 in PBS (pH 7.4) for 1 hour at room temperature, and thereafter with the primary antibody for 24–48 hours at +4°C in BS at a 1/1000 dilution. After washing in PBS, slices were incubated with the biotin-conjugated secondary antibody (1/4000) in BS, rinsed with PBS, and incubated with the avidin-peroxidase conjugate in BS for 1 hour at room temperature. The staining was detected using 3,3-diaminobenzidinetetrahydrochloride as a chromogen. -
Immunocytochemistry/ Immunofluorescence - Anti-Cannabinoid Receptor I antibody (ab23703)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of mouse cultured hippocampal neurons, staining Cannabinoid Receptor I with ab23703.
Cells were fixed with paraformaldehyde, permeabilized with 0.05% Saponin and blocked with 10% fetal calf serum for 2 hous at 4°C. Samples were incubated with primary antibody (1/200 in 0.05% Saponin + fetal calf serum) for 12 hours at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Courtesy of an annonymous Abreviewab23703 staining Cannabinoid Receptor I in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Freundt-Revilla J et al. Spatial distribution of cannabinoid receptor type 1 (CB1) in normal canine central and peripheral nervous system. PLoS One 12:e0181064 (2017). IHC-P, ICC/IF ; Dog . Read more (PubMed: 28700706) »
- Mendizabal-Zubiaga J et al. Cannabinoid CB1 Receptors Are Localized in Striated Muscle Mitochondria and Regulate Mitochondrial Respiration. Front Physiol 7:476 (2016). Read more (PubMed: 27826249) »
レビューと Q&A
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