Anti-cAMP 抗体 (ab832)

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Key features and details

  • Sheep polyclonal to cAMP
  • Suitable for: ELISA, RIA
  • Reacts with: Species independent
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-cAMP antibody
    cAMP 一次抗体 製品一覧

  • 製品の詳細

    Sheep polyclonal to cAMP

  • 由来種

    Sheep

  • 特異性

    This antibody reacts with cyclic AMP and to lesser degrees with other 5’ nucleotides.

  • アプリケーション

    適用あり: ELISA, RIAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Succinylated cAMP conjugated to bovine serum albumin.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.01% Sodium azide
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
This antibody may be used in radioimmunoassays to detect and quantitate cAMP; 50% binding is achieved with a 1:1,000 dilution.

 

ターゲット情報

  • 関連性

    Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.

  • 細胞内局在

    Secreted

  • 別名

    • 3' 5' cyclic adenosine monophosphate antibody
    • Cyclic adenosine monophosphate antibody
    • Cyclic AMP antibody

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab832 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab832 は 2 報の論文で使用されています。

  • Sweeny L  et al. Evaluation of tyrosine receptor kinases in the interactions of head and neck squamous cell carcinoma cells and fibroblasts. Oral Oncol 48:1242-9 (2012). PubMed: 22795534
  • Parro V  et al. A microbial oasis in the hypersaline Atacama subsurface discovered by a life detector chip: implications for the search for life on Mars. Astrobiology 11:969-96 (2011). PubMed: 22149750

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Is it known if this antibody will cross react with other cyclic nucleotides, such as cCMP?

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Answer

ab832 may cross-react with other 5'-nucleotides, however sufficient dilution can eliminate this cross-reactivity while retaining reactivity with cAMP. Working dilution should be optimized by the end user.

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Question

ab24851, ab12414, ab2345, and ab832 (cAMP antibodies) I've been performing a comprehensive study on available cAMP antibodies. Can you please indicate whether these antibodies have been produced/originate before or after 2003?

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Answer

Thank you for your enquiry. I have received replies from the four product originators. Here is the information that you requested for: ab12414 - First lots were sold in 1987 so before 2003. ab832 - Definitely before 2003. ab2345 - Not certain but would say after 2003. ab24851 - After 2003. Please don't hesitate to contact us again if you have further questions.

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