製品の概要
-
製品名Anti-CAMKIV antibody
CAMKIV 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CAMKIV
-
由来種Rabbit
-
アプリケーション適用あり: IHC-Fr, WB, ICC/IFmore details
-
種交差性交差種: Mouse, Rat, Dog, Human, Xenopus laevis, Non human primates, Plants, Turtle
-
免疫原
Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
Sequence:VEKGYYSERDAADAVKQ
(Peptide available asab5859) -
ポジティブ･コントロール
- WB: HeLa cell lysate.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab3557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).Can be blocked with CAMKIV peptide (ab5859). This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
|
| ICC/IF | Use at an assay dependent concentration. PubMed: 18802105 |
ターゲット情報
-
機能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
-
組織特異性Expressed in epithelial ovarian cancer tissue.
-
配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
-
細胞内局在Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
- Information by UniProt
-
参照データベース
- Entrez Gene: 814 Human
- Entrez Gene: 12326 Mouse
- Entrez Gene: 25050 Rat
- Omim: 114080 Human
- SwissProt: Q16566 Human
- SwissProt: P08414 Mouse
- SwissProt: P13234 Rat
- Unigene: 591269 Human
see all -
別名
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
画像
-
Western blot - Anti-CAMKIV antibody (ab3557)ab3557 at a 0.5µg/ml concentration staining 55 kDa CaMKIV in rat brain extract by Western blot.
プロトコール
参考文献
This product has been referenced in:
- Park D & Chang S Soluble Aß1-42increases the heterogeneity in synaptic vesicle pool size among synapses by suppressing intersynaptic vesicle sharing. Mol Brain 11:10 (2018). Read more (PubMed: 29463281) »
- Chen CM et al. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice. Proc Natl Acad Sci U S A 114:E619-E628 (2017). Read more (PubMed: 28069946) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"