Anti-CAMKIV 抗体 (ab3557)
Key features and details
- Rabbit polyclonal to CAMKIV
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CAMKIV antibody
CAMKIV 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CAMKIV -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
Sequence:VEKGYYSERDAADAVKQ
(Peptide available asab5859) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 2 µg/ml.
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| IHC-P |
1/20 - 1/100.
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| WB | (3) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
| 特記事項 |
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ICC
Use a concentration of 2 µg/ml. |
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IHC-P
1/20 - 1/100. |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
ターゲット情報
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機能
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. -
組織特異性
Expressed in epithelial ovarian cancer tissue. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. -
細胞内局在
Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 814 Human
- Entrez Gene: 12326 Mouse
- Entrez Gene: 25050 Rat
- Omim: 114080 Human
- SwissProt: Q16566 Human
- SwissProt: P08414 Mouse
- SwissProt: P13234 Rat
- Unigene: 591269 Human
see all -
別名
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
画像
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Immunocytochemistry - Anti-CAMKIV antibody (ab3557)
Immunofluorescence analysis of CaM Kinase IV in SH-SY5Y cells using ab3557. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab3557 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Alexa Fluor® 488 conjugate Goat anti-Rabbit IgG (H+L) at a 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (ab3557)Immunohistochemistry analysis of CaM Kinase IV using ab3557 at 1/100 dilution for 1 hour at 37ºC showing staining in the cytoplasm and nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left) followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (ab3557)Immunohistochemistry analysis of CaM Kinase IV using ab3557 at 1/20 dilution for 1 hour at 37ºC showing staining in the cytoplasm and nucleus of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature.
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Western blot - Anti-CAMKIV antibody (ab3557)All lanes : Anti-CAMKIV antibody (ab3557) at 1 µg/ml
Lane 1 : Mouse Brain tissue extract
Lane 2 : Rat Brain tissue extract
Lane 3 : Rat Liver tissue extract
Lane 4 : Rat Kidney tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution
Predicted band size: 52 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?A 59 kDa band corresponding to CaMKIV was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be CaMKIV negative.
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Western blot - Anti-CAMKIV antibody (ab3557)All lanes : Anti-CAMKIV antibody (ab3557) at 1 µg/ml
Lane 1 : PC12
Lane 2 : Rat Brain
Lane 3 : Mouse Brain
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 52 kDaA ~ 59 kDa band corresponding to CaM Kinase IV was observed across cell lines and tissues tested. Additionally ~25 kDa band was observed in PC12 cell line.
Blocking buffer: 5 % skimmed milk
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab3557 は 9 報の論文で使用されています。
- Zhang XZ et al. Mechanism of AMPK-mediated apoptosis of rat gastric smooth muscle cells under high glucose condition. Biosci Rep 39:N/A (2019). PubMed: 31769476
- Park D & Chang S Soluble Aß1-42increases the heterogeneity in synaptic vesicle pool size among synapses by suppressing intersynaptic vesicle sharing. Mol Brain 11:10 (2018). PubMed: 29463281
- Ramos-Fernández E et al. Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical Signaling. Mol Neurobiol N/A:N/A (2018). PubMed: 29967987
- Chen CM et al. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice. Proc Natl Acad Sci U S A 114:E619-E628 (2017). PubMed: 28069946
- Kang X et al. The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development. Nat Cell Biol 17:665-77 (2015). WB . PubMed: 25915125
- Zheng Z et al. Oligomeric amyloid-{beta} inhibits the proteolytic conversion of brain-derived neurotrophic factor (BDNF), AMPA receptor trafficking, and classical conditioning. J Biol Chem 285:34708-17 (2010). WB ; Turtle . PubMed: 20807770
- Toyoda H et al. Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice. Mol Brain 3:27 (2010). IHC . PubMed: 20846411
- Zheng Z & Keifer J PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning. J Neurophysiol 101:2539-49 (2009). WB ; Turtle . PubMed: 19261706
- Zhang X et al. Calcium/calmodulin-dependent protein kinase (CaMK) IV mediates nucleocytoplasmic shuttling and release of HMGB1 during lipopolysaccharide stimulation of macrophages. J Immunol 181:5015-23 (2008). WB, ICC/IF ; Human . PubMed: 18802105