Application
Western blot
Sample
Rat Cell lysate - whole cell (primary culture cortex cell)
Gel Running Conditions
Non-reduced Denaturing (15% SDS-PAGE)
Loading amount
15 µg
Treatment
RIPA & 1%Protease inhibitor for 15min on ice
Specification
primary culture cortex cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: ZSJQ IgG/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 50 kDa
Additional data
Additional Notes
We found two bands, but the upper band was blurred, and the lower band glowed more clearly, at 50 kd.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 08 2018