Anti-CamKII gamma 抗体 [8G10C1] (ab201966)
Key features and details
- Mouse monoclonal [8G10C1] to CamKII gamma
- Suitable for: IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CamKII gamma antibody [8G10C1]
CamKII gamma 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8G10C1] to CamKII gamma -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant fragment corresponding to Human CamKII gamma aa 300-500. Expressed in E. Coli.
Database link: Q13555-1 -
ポジティブ・コントロール
- IHC-P: Human prostate cancer and bladder cancer tissues. ICC/IF: Jurkat cells. WB: PC12, Jurkat, T47D, HeLa and HepG2 cell lysates; Human recombinant CamKII gamma (aa322-481) protein; Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR280299-5 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
8G10C1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201966の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/1000.
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 63 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 63 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 818 Human
- Entrez Gene: 171140 Rat
- Omim: 602123 Human
- SwissProt: Q13555 Human
- SwissProt: P11730 Rat
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別名
- CaM kinase II antibody
- CaM kinase II gamma chain antibody
- CaM kinase II subunit gamma antibody
see all
画像
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All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAMK2G knockout HeLa cell lysate
Lane 3 : T-47D cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaLanes 1-3: Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.
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Flow cytometric analysis of Jurkat cells labeling CamKII gamma with ab201966 at 1/200 dilution (green). Negative control (red).
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All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution
Lane 1 : PC12 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : T47D cell lysate
Lane 4 : HepG2 cell lysate
Predicted band size: 63 kDa -
All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 63 kDa -
Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution + Human recombinant CamKII gamma (aa322-481) protein
Predicted band size: 63 kDa(Expected MW is 44 kDa)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab201966 は 1 報の論文で使用されています。
- Boudreault S et al. Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components. Nucleic Acids Res 50:5263-5281 (2022). PubMed: 35489070