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Anti-CaMKII 抗体 [6G9] (ab22609)

Reviews (6)Q&A (2)References (64)
Western blot - Anti-CaMKII antibody [6G9] (ab22609)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [6G9] (ab22609)

Key features and details

  • Mouse monoclonal [6G9] to CaMKII
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CaMKII antibody [6G9]
    CaMKII 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [6G9] to CaMKII

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow

  • 免疫原

    Full length native protein (purified) corresponding to Rat CaMKII.

  • ポジティブ･コントロール

    • WB: Human, rat, and mouse whole brain tissue lysates; IHC-P: Rat normal brain FFPE tissue sections.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22609の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB (4)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
特記事項
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

ターゲット情報

画像

  • Western blot - Anti-CaMKII antibody [6G9] (ab22609)
    Western blot - Anti-CaMKII antibody [6G9] (ab22609)
    All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Human) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [6G9] (ab22609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [6G9] (ab22609)

    IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

参考文献 (64)

ab22609 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22609 は 64 報の論文で使用されています。

  • Jiang Y  et al. Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway. FEBS Open Bio 11:75-84 (2021). PubMed: 33125833
  • Lu J  et al. The Modulatory Effect of Motor Cortex Astrocytes on Diabetic Neuropathic Pain. J Neurosci 41:5287-5302 (2021). PubMed: 33753547
  • Liu Q  et al. Minocycline alleviates the symptoms of morphine withdrawal via the CaMKII-Ras-ERK signaling pathway. Neurosci Lett 752:135825 (2021). PubMed: 33727130
  • Varshney R  et al. Myocardial Hypertrophy and Compensatory Increase in Systolic Function in a Mouse Model of Oxidative Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33670798
  • Coviello S  et al. PSA Depletion Induces the Differentiation of Immature Neurons in the Piriform Cortex of Adult Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34072166
View all Publications for this product

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1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-CaMKII antibody [6G9]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Jan 31 2018

Western blot abreview for Anti-CaMKII antibody [6G9]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Jan 31 2018

Western blot abreview for Anti-CaMKII antibody [6G9]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Jan 31 2018

Western blot abreview for Anti-CaMKII antibody [6G9]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (15% PAGE)
Loading amount
25 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

投稿 Jan 31 2018

Immunohistochemistry (Frozen sections) abreview for Anti-CaMKII antibody [6G9]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Medaka fish Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 03 2017

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CaMKII antibody [6G9]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Dorsal Raphe Nucleus)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Specification
Dorsal Raphe Nucleus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formalin
Read More

Abcam user community

Verified customer

投稿 Oct 26 2015

Question

Inquiry: I am using the following 3 antibodies (ab22609, ab32678, ab138392), but I am unable to get a clear band at the specified KDa molecular weight. The protein bands of interest are always at a higher MW, with alot of non-specific bands showing. Could you please tell me the protein sequence for the above 3 antibodies so I can BLAST it to look at the sequence homolgy with Bovine Tissues, and find out what the non-specific bands are. if for any reason you are unable to provide the sequence could you please BLAST it on my behalf and send me a report? Many Thanks.

Read More
Answer

Thank you for contacting us.

The Bovine species for all of these antibodies is predicted. I am sorry we do not hold any bovine specific data.

The immunogen of these antibodies are as follows;

- ab22609; Partially purified full length native rat protein.
The full length protein between human and bovine is more than 90% homologous.

- ab138392; Synthetic phosphopeptide corresponding to residues surrounding Threonine 287 from the internal region of Human CaMK2 beta gamma delta.

- ab32678; Synthetic phosphopeptide corresponding to amino acids surrounding phospho-286 from Rat brain CaMKII.

ab138392 and ab32678 are the phospho specific antibodies. Milk as blocking agent is not recommended for these.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi, I am interested in ELISA kit for the human CAMKII alpha (ab2724) . There is no ready to use kit and I wonder if you can help me to choose anitbodies for ELISA sandwich. I have already use the two of your products for Western Blot. ab22609 and ab 2724 that is my target and ab60899 for quantifieng the western blot. I would realy appreciate if you help me to choose the antibodies for running Sandwich ELISA and the plates that need for runing my expereiments. Best Regards

Read More
Answer

Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. At this moment we do not have tested CamKII antibody pairs that can be used in sELISA. However based on the requirement of different epitope specificity I can recommend using ab54925 or ab54926 with ab89197 or ab92332. The antibodies are specific to different part of antigens so these can be used in sELISA. Please be advised that sELISA is not a tested application so these products are not covered under Abpromise guarantee. However I can certainly offer you Abreview discount codes as these products are eligible. The discount codes will give you next primary antibody free of charge if you test these antibodies in sELISA and submit an Abreview. You may have to use one of these antibodies as detection or capture so I can provide you 2 discount codes for 2 products. Let me know if you are interested in obtaining this offer I will then be sending you the discount codes right away. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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