Anti-CaMKII alpha (phospho T286) 抗体 [EPR1830] - BSA and Azide free (ab248035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1830] to CaMKII alpha (phospho T286) - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-CaMKII alpha (phospho T286) antibody [EPR1830] - BSA and Azide free
CaMKII alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1830] to CaMKII alpha (phospho T286) - BSA and Azide free -
由来種
Rabbit -
特異性
Due to the homology between the immunogen peptide and other CAMKII isoforms, this antibody may cross react with CAMKII delta (KCC2D, 93% homology) and CAMKII gamma (KCC2G, 79% homology).
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse, Rat
非交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248035 is the carrier-free version of ab124880.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 3.60 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1830 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248035の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
- Unigene: 131530 Mouse
- Unigene: 107499 Rat
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別名
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
画像
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All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880) at 1/4000 dilution
Lane 1 : Rat brain tissue lysate (untreated membrane)
Lane 2 : Rat brain tissue lysate (Lambda phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50,54 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This data was developed using ab124880, the same antibody clone in a different buffer formulation.
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All lanes : Anti-CaMKII alpha (phospho T286) antibody [EPR1830] (ab124880) at 1/4000 dilution
Lane 1 : Mouse brain tissue lysate (untreated membrane)
Lane 2 : Mouse brain tissue lysate (Lambda phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50,54 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This data was developed using ab124880, the same antibody clone in a different buffer formulation.
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Immunohistochemical analysis of Paraffin-embedded sections Rat Cerebellum tissue labelling CaMKII (pT286) with ab124880 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)(Ready to use). Rat cerebellum without Lambda Protein Phosphatase treatment staining on Rat Cerebellum is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)(Ready to use).
Positive staining on rat cerebellum without Lambda Protein Phosphatase treatment (image A); No signal was detected when tissues were treated with Lambda Protein Phosphatase (image B).The section was incubated with ab124880 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.This data was developed using ab124880, the same antibody clone in a different buffer formulation.
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Immunohistochemical analysis of Paraffin-embedded sections Mouse Cerebellum tissue labelling CaMKII (pT286) with ab124880 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Mouse cerebellum without Lambda Protein Phosphatase treatment staining on Mouse Cerebellum is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (abab209101) at Ready to use dilution.
Positive staining on mouse cerebellum without Lambda Protein Phosphatase treatment (image A); No signal was detected when tissues were treated with Lambda Protein Phosphatase (image B).The section was incubated with ab124880 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.This data was developed using ab124880, the same antibody clone in a different buffer formulation.
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This data was developed using ab124880, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248035 は論文での使用が確認できていません。