Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1830] to CaMKII alpha + CaMKII beta (phospho T286) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
製品名Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] - BSA and Azide free
CaMKII alpha + CaMKII beta (phospho T286) 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1830] to CaMKII alpha + CaMKII beta (phospho T286) - BSA and Azide free
Due to the homology between the immunogen peptide and other CAMKII isoforms, this antibody may cross react with CAMKII delta (KCC2D, 93% homology) and CAMKII gamma (KCC2G, 79% homology).
アプリケーション適用あり: WBmore details
適用なし: ICC,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human CaMKII alpha + CaMKII beta (phospho T286) aa 250-350. The exact sequence is proprietary.
Ab248035 is the carrier-free version of ab124880. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248035 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 3.60 x 10 -11 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 45-70 kDa (predicted molecular weight: 54 kDa).|
関連性Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
All lanes : Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody [EPR1830] (ab124880) at 1/1000 dilution
Lane 1 : Rat brain lysate untreated
Lane 2 : Rat brain lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
This data was developed using ab124880, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.