Anti-Calsequestrin 1 抗体 [EPR15227(B)] (ab191564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15227(B)] to Calsequestrin 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Calsequestrin 1 antibody [EPR15227(B)]
Calsequestrin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15227(B)] to Calsequestrin 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human, mouse and rat skeletal muscle lysates; Human and rat skeletal muscle tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15227(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 63 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. Also binds laminin. -
配列類似性
Belongs to the calsequestrin family. -
細胞内局在
Sarcoplasmic reticulum lumen. Mitochondrion. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial according to PubMed:7945294. - Information by UniProt
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参照データベース
- Entrez Gene: 844 Human
- Entrez Gene: 12372 Mouse
- Entrez Gene: 686019 Rat
- Omim: 114250 Human
- SwissProt: P31415 Human
- SwissProt: O09165 Mouse
- SwissProt: P19633 Rat
- Unigene: 632476 Human
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別名
- Calmitin antibody
- Calmitine antibody
- Calsequestrin 1 (fast twitch, skeletal muscle) antibody
see all
画像
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Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution + Human skeletal muscle tissue lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded, Human skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded, rat skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab191564 は 4 報の論文で使用されています。
- Endo Y et al. Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility. Nat Commun 13:3403 (2022). PubMed: 35697689
- Wang Z et al. A Temporal Examination of Cytoplasmic Ca2 + Levels, Sarcoplasmic Reticulum Ca2 + Levels, and Ca2 + -Handling-Related Proteins in Different Skeletal Muscles of Hibernating Daurian Ground Squirrels. Front Physiol 11:562080 (2020). PubMed: 33192559
- Jannas-Vela S et al. Resting metabolic rate and skeletal muscle SERCA and Na+ /K+ ATPase activities are not affected by fish oil supplementation in healthy older adults. Physiol Rep 8:e14408 (2020). PubMed: 32342642
- Miotto PM & Holloway GP Exercise-induced reductions in mitochondrial ADP sensitivity contribute to the induction of gene expression and mitochondrial biogenesis through enhanced mitochondrial H2O2 emission. Mitochondrion 46:116-122 (2019). PubMed: 29588219