Anti-Calretinin 抗体 (ab702)

Reviews (4)Q&A (3)References (37)

製品の概要

  • 製品名

    Anti-Calretinin antibody
    Calretinin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Calretinin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Chicken, Cat, Dog, Xenopus laevis

  • 免疫原

    Full length protein corresponding to Calretinin.

  • ポジティブ･コントロール

    • IHC-P: Mesothelioma. IHC-Fr: Mouse brain, zebrafish retinal tissue and sea cucumber radial nerve cord tissue.

  • 特記事項

    Contains a proprietary preservative that is not sodium azide or thimerosal.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.30
    Preservative: 0.09% Sodium azide
    Constituents: PBS, Carrier protein

    Van Gogh Yellow Diluent

  • 精製度

    Whole antiserum

  • ポリ/モノ

    ポリクローナル

  • ミエローマ

    unknown

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.
IHC-Fr 1/100. PubMed: 20504962

Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

    Paraffin embedded mesothelioma (human) stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)This image is courtesy of an Abreview submitted by Bettina Hoerz

    ab702 staining Calretinin in mouse brain tissue sections in immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)Adapted with permission from Cerveny KL et al, Development. 2010 Jul;137(13):2107-15. Epub 2010 May 26, Fig S1.

    ab702 staining Calretinin (green) in zebrafish retinal cells by immunohistochemistry (frozen sections). Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat. Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped. Original article: http://dev.biologists.org/content/137/13/2107.long

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)Image from Díaz-Balzac CA et al., PLoS One. 2012;7(3):e32689. Epub 2012 Mar 7. Fig 1.; doi:10.1371/journal.pone.0032689; March 7, 2012, PLoS ONE 7(3): e32689.

    Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.

プロトコール

参考文献

This product has been referenced in:

  • Akgül G  et al. The Role of AMPARs in the Maturation and Integration of Caudal Ganglionic Eminence-Derived Interneurons into Developing Hippocampal Microcircuits. Sci Rep 9:5435 (2019). Read more (PubMed: 30931998) »
  • Sawant OB  et al. The circadian clock gene Bmal1 is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina. FASEB J N/A:fj201801832RR (2019). Read more (PubMed: 31002540) »
See all 37 Publications for this product

ab702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calretinin antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain(Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X
Specification
Brain(Hippocampus)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
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Mr. J. Y. Seo

Verified customer

投稿 Apr 03 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody

 Below Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lung)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Mrs. EUN JEUNG KIM

Verified customer

投稿 Jan 12 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Permeabilization
Yes - Triton X-100 in Blocking Solution
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative
Read More

Abcam user community

Verified customer

投稿 Jul 31 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample
Mouse Tissue sections (frozen brain section)
Specification
frozen brain section
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Mrs. Bettina Hoerz

Verified customer

投稿 Jan 06 2014

Question

I would like to know the expiration date of the antibody ab702, lot number 877133.

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Answer



We do not have specific use by dates for our products.

If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

Please review and follow carefully the storage instructions on the individual datasheets.

For more tips on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols).

We suggest the following approximate use by dates for antibodies depending on which temperature they are recommended to be stored at:

room temperature: 1 week

+ 4°C: 1 year

- 20°C: 5 years

- 80°C: 10 years

Other product types are also guaranteed for 1 year. However, specific storage instructions are provided on datasheets and protocol booklets. Sometimes kit components will have a shorter shelf life once reconstituted or prepared and details are provided in protocol booklets for kits.

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Question

Estimado:
Estuve conversando con el cliente, las publicaciones que necesita es relacionado con las referencias en donde se haya utilizado el mesotelioma como control positivo para Calretinina (ab702).
Espero que me puedas ayudar con esto.
Saludos cordiales

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Answer

Gracias por tu respuesta.

Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas técnicas encuentre exactamente lo que está buscando. El enlace a las distintas alternativas es el siguiente:

https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=Calretinin

Espero que consiga la información que necesita. Para cualquier otra información, no dudes en volvernos a contactar.

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Question

Buenas tardes, podrian por favor enviarme los articulos referenciados en la ficha tecnica de ab702? No consigo acceder a ellos.
Muchas gracias.

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Answer

Gracias por contactarnos.


Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuación le llevan directamente al artículo:

http://www.jneurosci.org/content/31/25/9368.long

http://dev.biologists.org/content/137/13/2107.long

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517219/?tool=pubmed

http://cercor.oxfordjournals.org/content/18/7/1604.long

http://toxsci.oxfordjournals.org/content/89/1/314.long

El resto de artículos no son de libre acceso, es necesario suscribirse a la revista para poder acceder a sus publicaciones. Si no son miembros de la revista, no podrán acceder a la misma online, y me temo que solo podrán consultar los abstracts.

Espero que esta información sea de utilidad, en caso contrario, no dudes en volverme a contactar.

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