Anti-Calretinin 抗体 (ab702)

Reviews (4)Q&A (3)References (47)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

    Key features and details

    • Rabbit polyclonal to Calretinin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Calretinin antibody [EP1798] (ab92341)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Calretinin antibody
      Calretinin 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Calretinin

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Chicken, Cat, Dog, Xenopus laevis

    • 免疫原

      Full length protein corresponding to Calretinin.

    • 特記事項

      Contains a proprietary preservative that is not sodium azide or thimerosal.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.09% Sodium azide
      Constituents: PBS, Carrier protein

      Van Gogh Yellow Diluent
    • Concentration information loading...

    • 精製度

      Whole antiserum

    • ポリ/モノ

      ポリクローナル

    • ミエローマ

      unknown

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab702の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (1)
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.
    特記事項
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

    ターゲット情報

    • 機能

      Calretinin is a calcium-binding protein which is abundant in auditory neurons.

    • 組織特異性

      Brain.

    • 配列類似性

      Belongs to the calbindin family.
      Contains 6 EF-hand domains.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 29 kDa calbindin antibody
        • CAB 29 antibody
        • CAB29 antibody
        • CAL 2 antibody
        • CAL2 antibody
        • CALB 2 antibody
        • CALB2 antibody
        • CALB2_HUMAN antibody
        • Calbindin 2 29kDa antibody
        • Calbindin 2 antibody
        • Calbindin D29K antibody
        • Calbindin2 antibody
        • Calretinin antibody
        • CR antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

        Paraffin embedded mesothelioma (human) stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

      参考文献 (47)

      ab702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab702 は 47 報の論文で使用されています。

      • Ribeiro J  et al. Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors. Cell Rep 35:109022 (2021). PubMed: 33882303
      • Khatib TZ  et al. Receptor-ligand supplementation via a self-cleaving 2A peptide-based gene therapy promotes CNS axonal transport with functional recovery. Sci Adv 7:N/A (2021). PubMed: 33789891
      • Prahst C  et al. Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy. Elife 9:N/A (2020). PubMed: 32073398
      • Kim S  et al. Dynamic Fas signaling network regulates neural stem cell proliferation and memory enhancement. Sci Adv 6:eaaz9691 (2020). PubMed: 32494656
      • Emam A  et al. Retinal morphology in Astyanax mexicanus during eye degeneration. J Comp Neurol 528:1523-1534 (2020). PubMed: 31811648
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-7 of 7 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calretinin antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Brain(Hippocampus))
      Antigen retrieval step
      None
      Permeabilization
      Yes - 0.2% Triton X
      Specification
      Brain(Hippocampus)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Mr. J. Y. Seo

      Verified customer

      投稿 Apr 03 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody

       Below Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Dog Tissue sections (lung)
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Specification
      lung
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Mrs. EUN JEUNG KIM

      Verified customer

      投稿 Jan 12 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Fukomys anselli Tissue sections (Retina)
      Permeabilization
      Yes - Triton X-100 in Blocking Solution
      Specification
      Retina
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
      Fixative
      Davidson's Fixative
      Read More

      Abcam user community

      Verified customer

      投稿 Jul 31 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
      Sample
      Mouse Tissue sections (frozen brain section)
      Specification
      frozen brain section
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More

      Mrs. Bettina Hoerz

      Verified customer

      投稿 Jan 06 2014

      Question

      I would like to know the expiration date of the antibody ab702, lot number 877133.

      Read More
      Answer



      We do not have specific use by dates for our products.

      If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

      Please review and follow carefully the storage instructions on the individual datasheets.

      For more tips on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols).

      We suggest the following approximate use by dates for antibodies depending on which temperature they are recommended to be stored at:

      room temperature: 1 week

      + 4°C: 1 year

      - 20°C: 5 years

      - 80°C: 10 years

      Other product types are also guaranteed for 1 year. However, specific storage instructions are provided on datasheets and protocol booklets. Sometimes kit components will have a shorter shelf life once reconstituted or prepared and details are provided in protocol booklets for kits.

      Read More

      Question

      Estimado:
      Estuve conversando con el cliente, las publicaciones que necesita es relacionado con las referencias en donde se haya utilizado el mesotelioma como control positivo para Calretinina (ab702).
      Espero que me puedas ayudar con esto.
      Saludos cordiales

      Read More
      Answer

      Gracias por tu respuesta.

      Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

      Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas técnicas encuentre exactamente lo que está buscando. El enlace a las distintas alternativas es el siguiente:

      https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=Calretinin

      Espero que consiga la información que necesita. Para cualquier otra información, no dudes en volvernos a contactar.

      Read More

      Question

      Buenas tardes, podrian por favor enviarme los articulos referenciados en la ficha tecnica de ab702? No consigo acceder a ellos.
      Muchas gracias.

      Read More
      Answer

      Gracias por contactarnos.


      Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuación le llevan directamente al artículo:

      http://www.jneurosci.org/content/31/25/9368.long

      http://dev.biologists.org/content/137/13/2107.long

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517219/?tool=pubmed

      http://cercor.oxfordjournals.org/content/18/7/1604.long

      http://toxsci.oxfordjournals.org/content/89/1/314.long

      El resto de artículos no son de libre acceso, es necesario suscribirse a la revista para poder acceder a sus publicaciones. Si no son miembros de la revista, no podrán acceder a la misma online, y me temo que solo podrán consultar los abstracts.

      Espero que esta información sea de utilidad, en caso contrario, no dudes en volverme a contactar.

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