Anti-Calretinin 抗体 (ab702)

Reviews (4)Q&A (3)References (44)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

    Key features and details

    • Rabbit polyclonal to Calretinin
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Calretinin antibody
      Calretinin 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Calretinin

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat, Human
      交差が予測される動物種: Chicken, Cat, Dog, Xenopus laevis

    • 免疫原

      Full length protein corresponding to Calretinin.

    • 特記事項

      Contains a proprietary preservative that is not sodium azide or thimerosal.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.09% Sodium azide
      Constituents: PBS, Carrier protein

      Van Gogh Yellow Diluent
    • Concentration information loading...

    • 精製度

      Whole antiserum

    • ポリ/モノ

      ポリクローナル

    • ミエローマ

      unknown

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab702 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

    ターゲット情報

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)

      Paraffin embedded mesothelioma (human) stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

    参考文献 (44)

    ab702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab702 は 44 報の論文で使用されています。

    • Prahst C  et al. Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy. Elife 9:N/A (2020). PubMed: 32073398
    • Kim S  et al. Dynamic Fas signaling network regulates neural stem cell proliferation and memory enhancement. Sci Adv 6:eaaz9691 (2020). PubMed: 32494656
    • Akgül G  et al. The Role of AMPARs in the Maturation and Integration of Caudal Ganglionic Eminence-Derived Interneurons into Developing Hippocampal Microcircuits. Sci Rep 9:5435 (2019). PubMed: 30931998
    • Sawant OB  et al. The circadian clock gene Bmal1 is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina. FASEB J N/A:fj201801832RR (2019). PubMed: 31002540
    • Szymkowicz DB  et al. Exposure to arsenic during embryogenesis impairs olfactory sensory neuron differentiation and function into adulthood. Toxicology 420:73-84 (2019). PubMed: 30978373
    View all Publications for this product

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calretinin antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain(Hippocampus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain(Hippocampus)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. J. Y. Seo

    Verified customer

    投稿 Apr 03 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calretinin antibody

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (lung)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    lung
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. EUN JEUNG KIM

    Verified customer

    投稿 Jan 12 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Fukomys anselli Tissue sections (Retina)
    Permeabilization
    Yes - Triton X-100 in Blocking Solution
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Davidson's Fixative
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 31 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Calretinin antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
    Sample
    Mouse Tissue sections (frozen brain section)
    Specification
    frozen brain section
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Bettina Hoerz

    Verified customer

    投稿 Jan 06 2014

    Question

    I would like to know the expiration date of the antibody ab702, lot number 877133.

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    Answer



    We do not have specific use by dates for our products.

    If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

    Please review and follow carefully the storage instructions on the individual datasheets.

    For more tips on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols).

    We suggest the following approximate use by dates for antibodies depending on which temperature they are recommended to be stored at:

    room temperature: 1 week

    + 4°C: 1 year

    - 20°C: 5 years

    - 80°C: 10 years

    Other product types are also guaranteed for 1 year. However, specific storage instructions are provided on datasheets and protocol booklets. Sometimes kit components will have a shorter shelf life once reconstituted or prepared and details are provided in protocol booklets for kits.

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    Question

    Estimado:
    Estuve conversando con el cliente, las publicaciones que necesita es relacionado con las referencias en donde se haya utilizado el mesotelioma como control positivo para Calretinina (ab702).
    Espero que me puedas ayudar con esto.
    Saludos cordiales

    Read More
    Answer

    Gracias por tu respuesta.

    Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

    Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas técnicas encuentre exactamente lo que está buscando. El enlace a las distintas alternativas es el siguiente:

    https://www.abcam.com/index.html?pageconfig=searchresults&pt=1&search=Calretinin

    Espero que consiga la información que necesita. Para cualquier otra información, no dudes en volvernos a contactar.

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    Question

    Buenas tardes, podrian por favor enviarme los articulos referenciados en la ficha tecnica de ab702? No consigo acceder a ellos.
    Muchas gracias.

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    Answer

    Gracias por contactarnos.


    Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuación le llevan directamente al artículo:

    http://www.jneurosci.org/content/31/25/9368.long

    http://dev.biologists.org/content/137/13/2107.long

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517219/?tool=pubmed

    http://cercor.oxfordjournals.org/content/18/7/1604.long

    http://toxsci.oxfordjournals.org/content/89/1/314.long

    El resto de artículos no son de libre acceso, es necesario suscribirse a la revista para poder acceder a sus publicaciones. Si no son miembros de la revista, no podrán acceder a la misma online, y me temo que solo podrán consultar los abstracts.

    Espero que esta información sea de utilidad, en caso contrario, no dudes en volverme a contactar.

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