Anti-Calretinin 抗体 (ab702)
製品の概要
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製品名Anti-Calretinin antibody
Calretinin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Calretinin
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由来種Rabbit
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アプリケーション適用あり: IHC-P, IHC-Frmore details
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種交差性交差種: Mouse, Rat, Human, Zebrafish
交差が予測される動物種: Chicken, Cat, Dog, Xenopus laevis
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免疫原
Full length protein corresponding to Calretinin.
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ポジティブ･コントロール
- IHC-P: Mesothelioma. IHC-Fr: Mouse brain, zebrafish retinal tissue and sea cucumber radial nerve cord tissue.
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特記事項Contains a proprietary preservative that is not sodium azide or thimerosal.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.30
Preservative: 0.09% Sodium azide
Constituents: PBS, Carrier protein
Van Gogh Yellow Diluent -
精製度Whole antiserum
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ポリ/モノポリクローナル
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ミエローマunknown
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Unmasking reagent
アプリケーション
Our Abpromise guarantee covers the use of ab702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT. |
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| IHC-Fr | 1/100. PubMed: 20504962 Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%). |
ターゲット情報
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機能Calretinin is a calcium-binding protein which is abundant in auditory neurons.
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組織特異性Brain.
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配列類似性Belongs to the calbindin family.
Contains 6 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 396255 Chicken
- Entrez Gene: 794 Human
- Entrez Gene: 12308 Mouse
- Entrez Gene: 117059 Rat
- Entrez Gene: 393684 Zebrafish
- Omim: 114051 Human
- SwissProt: P07090 Chicken
- SwissProt: P22676 Human
see all -
別名
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab702)Paraffin embedded mesothelioma stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)This image is courtesy of an Abreview submitted by Bettina Hoerzab702 staining Calretinin in mouse brain tissue sections in immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)Adapted with permission from Cerveny KL et al, Development. 2010 Jul;137(13):2107-15. Epub 2010 May 26, Fig S1.ab702 staining Calretinin (green) in zebrafish retinal cells by immunohistochemistry (frozen sections). Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat. Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped. Original article: http://dev.biologists.org/content/137/13/2107.long
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Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody (ab702)Image from Díaz-Balzac CA et al., PLoS One. 2012;7(3):e32689. Epub 2012 Mar 7. Fig 1.; doi:10.1371/journal.pone.0032689; March 7, 2012, PLoS ONE 7(3): e32689.Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.
参考文献
This product has been referenced in:
- Mazzocchi AR et al. In vitro patient-derived 3D mesothelioma tumor organoids facilitate patient-centric therapeutic screening. Sci Rep 8:2886 (2018). Read more (PubMed: 29440675) »
- Zimmerman R et al. Repeated Prenatal Exposure to Valproic Acid Results in Auditory Brainstem Hypoplasia and Reduced Calcium Binding Protein Immunolabeling. Neuroscience 377:53-68 (2018). ICC/IF . Read more (PubMed: 29510209) »
