製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Calretinin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Chicken, Cat, Dog, Xenopus laevis
  • 免疫原

    Full length protein corresponding to Calretinin.

  • ポジティブ・コントロール
    • IHC-P: Mesothelioma. IHC-Fr: Mouse brain, zebrafish retinal tissue and sea cucumber radial nerve cord tissue.
  • 特記事項
    Contains a proprietary preservative that is not sodium azide or thimerosal.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.30
    Preservative: 0.09% Sodium azide
    Constituents: PBS, Carrier protein

    Van Gogh Yellow Diluent
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • ミエローマ
    unknown
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

IHC-Fr 1/100. PubMed: 20504962

Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).

ターゲット情報

画像

  • Paraffin embedded mesothelioma stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

  • ab702 staining Calretinin in mouse brain tissue sections in immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab702 staining Calretinin (green) in zebrafish retinal cells by immunohistochemistry (frozen sections). Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat. Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped. Original article: http://dev.biologists.org/content/137/13/2107.long

  • Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.

プロトコール

参考文献

This product has been referenced in:
  • Mazzocchi AR  et al. In vitro patient-derived 3D mesothelioma tumor organoids facilitate patient-centric therapeutic screening. Sci Rep 8:2886 (2018). Read more (PubMed: 29440675) »
  • Zang L  et al. Accessory ovarian steroid cell tumor producing testosterone and cortisol: A case report. Medicine (Baltimore) 96:e7998 (2017). IHC ; Human . Read more (PubMed: 28906379) »

See all 25 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain(Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X
Specification
Brain(Hippocampus)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mr. J. Y. Seo

Verified customer

投稿 Apr 03 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lung)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mrs. EUN JEUNG KIM

Verified customer

投稿 Jan 12 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Permeabilization
Yes - Triton X-100 in Blocking Solution
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative
Username

Abcam user community

Verified customer

投稿 Jul 31 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample
Mouse Tissue sections (frozen brain section)
Specification
frozen brain section
Permeabilization
No
Fixative
Paraformaldehyde
Username

Mrs. Bettina Hoerz

Verified customer

投稿 Jan 06 2014



We do not have specific use by dates for our products.

If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

Please review...

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Gracias por tu respuesta.

Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas ...

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Gracias por contactarnos.


Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuaci...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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