製品名Anti-Calreticulin antibody - ER Marker
Calreticulin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Calreticulin - ER Marker
アプリケーション適用あり: WBmore details
交差が予測される動物種: Mouse, Rat, Rabbit, Chinese hamster
- This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line); Jurkat (Human T cell lymphoblast-like cell line); HepG2 (Human hepatocellular liver carcinoma cell line); A431 (Human epithelial carcinoma cell line).
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab39897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).|
機能Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
配列類似性Belongs to the calreticulin family.
ドメインCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
細胞内局在Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
- Information by UniProt
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Calreticulin knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: NIH3T3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab39897 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab39897 was shown to recognize Calreticulin when Calreticulin knockout samples were used, along with additional cross-reactive bands. Wild-type and Calreticulin knockout samples were subjected to SDS-PAGE. ab39897 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Calreticulin antibody - ER Marker (ab39897) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
HepG2 whole cell lysate (ab7900)
Lane 4 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
The predicted band size is 48kDa based on Swiss-prot data, however a band of 55kDa is consistent with that observed in other commercially available antibodies to Calreticulin. This difference in size is likely to be the result of post-translational modification.