Application
Western blot
Sample
Arabidopsis thaliana Cell lysate - whole cell (cell culture)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
cell culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Pierce (31461): Goat Anti-Rabbit IgG (H+L) [F(ab')
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Dura
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 60, 70 kDa Non-specific: 85?, 40, 50, 55 kDa
Additional data
Additional Notes
Lane 1: Markers
Lane 2: whole cell lysate
Lane 3: 10,000g pellet
Lane 4: 100,000g pellet
Lane 5: 100,000g supernatant
PubMed ID
21166475: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Joshua Heazlewood
Verified customer
投稿 Jul 14 2011