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Anti-Calreticulin 抗体 - ER Marker (ab2907)

  • Datasheet
  • SDS
Reviews (16)Q&A (3)References (155)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

Key features and details

  • Rabbit polyclonal to Calreticulin - ER Marker
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Calreticulin antibody - ER Marker
    Calreticulin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Calreticulin - ER Marker
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Calreticulin.

  • ポジティブ・コントロール

    • WB: HL-60, LNCaP, HeLa and MCF-7 cell lysates; Mouse and rat liver tissue lysates; Mouse skeletal muscle whole cell lysate. ICC/IF: A431, HeLa, U2OS, HepG2 and HMVEC cells.
  • 特記事項

      

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Calreticulin protein (ab15729)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Recombinant Calreticulin protein (ab15729)

アプリケーション

Our Abpromise guarantee covers the use of ab2907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/1000.

ターゲット情報

  • 機能

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 配列類似性

    Belongs to the calreticulin family.
  • ドメイン

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 細胞内局在

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Target information above from: UniProt accession P27797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 811 Human
    • Entrez Gene: 12317 Mouse
    • Entrez Gene: 64202 Rat
    • Omim: 109091 Human
    • SwissProt: P27797 Human
    • SwissProt: P14211 Mouse
    • SwissProt: P18418 Rat
    • Unigene: 515162 Human
    • Unigene: 1971 Mouse
    • Unigene: 467043 Mouse
    • Unigene: 974 Rat
    see all
  • 別名

    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

画像

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
    All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : LNCaP cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF-7 cell lysate
    Lane 5 : Mouse liver tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Image from Yount JS et al, J Biol Chem. 2012 Jun 1;287(23):19631-41. Epub 2012 Apr 17, Fig 3. DOI 10.1074/jbc.M112.362095 June 1, 2012 The Journal of Biological Chemistry, 287, 19631-19641.
    ab2907 used at a 1/1000 dilution staining Calreticulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    HeLa cells were transfected overnight with empty vector or plasmids encoding the indicated IFITM3 constructs. Immunofluorescence with a-HA antibodies allowed IFITM3 visualization, and a-calreticulin staining allowed visualization of the ER. TOPRO-3 was used to visualize nuclei. Scale bars indicate 10 µm. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine.
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution

    All lanes : Whole cell lysate prepared from mouse skeletal muscle

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated mouse polyclonal to rabbit Ig at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (1:300) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemsitry/Immunofluorescence analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X for 30 minutes at room temperature. Cells were incubated with ab2907 (1:50) for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (1:300) in PBS and incubated for 30 minutes. Nuclei (blue) were stained with Hoechst 33342 dye (1µg/mL). Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (1:120) and nuclei (red) were stained with DRAQ5 (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labelling Calreticulin using ab2907.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous Abreview
    Immunofluorescence analysis of HepG2 cells, staining Calreticulin with ab2907.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% saponin) for 1 hour at 20°C. An AlexaFluor®647-conjugated donkey anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (155)

    ab2907 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2907 は 155 報の論文で使用されています。

    • Fu D  et al. T cell recruitment triggered by optimal dose platinum compounds contributes to the therapeutic efficacy of sequential PD-1 blockade in a mouse model of colon cancer. Am J Cancer Res 10:473-490 (2020). PubMed: 32195021
    • Yang J  et al. Dinaciclib prolongs survival in the LSL-KrasG12D/+ ; LSL-Trp53R172H/+ ; Pdx-1-Cre (KPC) transgenic murine models of pancreatic ductal adenocarcinoma. Am J Transl Res 12:1031-1043 (2020). PubMed: 32269732
    • Scherr AL  et al. Knockdown of Atg7 Induces Nuclear-LC3 Dependent Apoptosis and Augments Chemotherapy in Colorectal Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32046105
    • Song S  et al. Tetrahydrobenzimidazole TMQ0153 triggers apoptosis, autophagy and necroptosis crosstalk in chronic myeloid leukemia. Cell Death Dis 11:109 (2020). PubMed: 32034134
    • Pacheco A  et al. Mechanism and role of the intra-axonal Calreticulin translation in response to axonal injury. Exp Neurol 323:113072 (2020). PubMed: 31669485
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 19 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calreticulin antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (184A1 mammary epithelial)
    Permeabilization
    Yes - PBS + 0.25% Triton-X
    Specification
    184A1 mammary epithelial
    Blocking step
    (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 28 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calreticulin antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - 0.5% triton-X100
    Specification
    U2OS
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Lawrence Welch

    Verified customer

    投稿 Jun 16 2020

    Western blot abreview for Anti-Calreticulin antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela cells)
    Gel Running Conditions
    Non-reduced Denaturing (premade 4-12%)
    Loading amount
    15 µg
    Specification
    Hela cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Mrs. Federica Ferrentino

    Verified customer

    投稿 Mar 05 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calreticulin antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 25 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calreticulin antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 24 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calreticulin antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Drosophila melanogaster Cell (hemocyte (S2R+ cell))
    Permeabilization
    Yes - 0.1% TX-100
    Specification
    hemocyte (S2R+ cell)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Miguel Sanchez

    Verified customer

    投稿 Jul 18 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calreticulin antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2)
    Permeabilization
    Yes - Saponin 0.1%
    Specification
    HepG2
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 30 2012

    Question

    Does the storage buffer actually contain PBS? OR is it a whole ant-serum plus sodium azide?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2012

    Answer

    Thank you for contacting Abcam yesterday.
    The formulation for ab2907 should be 100 µl of antiserum containing 0.05% sodium azide. There is no PBS present.
    I will have our datasheets team update the webpage as soon as possible.
    I do apologize if this has created any issue.
    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Jul 12 2012

    Question

    I am looking for an Arabidopsis calreticulin antibody that
    is suitable for Western blot
    and/ or immunoprecipitation.
    Internet search engines show an old entry for your
    antibody ab2907,
    but when I open your homepage, this reference is no longer
    available.
    Is there any Arabidopsis calreticulin antibody available?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 16 2012

    Answer

    Thank you for contacting us.

    The antibody ab2907 was tried in Arabidopsis by a customer, and the results were submitted as an Abreview (2 out of 5 stars): https://www.abcam.com/Calreticulin-antibody-ER-Marker-ab2907.html&tab=abreviews&intabreviewid=24966

    Thus, the Arabidopsis data is on the datasheet, but due to the rating of the results we do not list Arabidopsis as tested species or guarantee the antibody for it.

    I have checked the homology of Arabidopsis with the human sequence and the homology is only 54% (isoform 1), 55% (isoform 2), and 49% (isoform 3) - thus very low. It is similar for the rabbit sequence: the homology between rabbit and Arabidopsis is also only 54%.
    We usually suggest a sequence homology of 85% or higher forpotentialcross-reactivity. Thus, in generell it would be difficult to find an antibody raised against human or rabbit to be able to crossreact with Arabidopsis.

    I might suggest to check http://www.biocompare.com for an antibody that was raised against an Arabidopsis sequence.

    I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 16 2012

    Western blot abreview for Anti-Calreticulin antibody - ER Marker

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Arabidopsis thaliana Cell lysate - whole cell (cell culture)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    cell culture
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Dr. Joshua Heazlewood

    Verified customer

    投稿 Jul 14 2011

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