Anti-Calreticulin 抗体 (ab4109)

Reviews (5)Q&A (5)References (4)

Key features and details

  • Goat polyclonal to Calreticulin
  • Suitable for: IHC-Fr, WB
  • Reacts with: Mouse, Rat, Rabbit, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Calreticulin antibody
    Calreticulin 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to Calreticulin

  • 由来種

    Goat

  • アプリケーション

    適用あり: IHC-Fr, WBmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Rabbit, Human

  • 免疫原

    Full length native protein (purified) corresponding to Rabbit Calreticulin. Purified rabbit skeletal muscle calreticulin

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4109の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (1)
Use at an assay dependent concentration.
WB (3)
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.

  • 配列類似性

    Belongs to the calreticulin family.

  • ドメイン

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.

  • 細胞内局在

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

参考文献 (4)

ab4109 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4109 は 4 報の論文で使用されています。

  • Sun Y  et al. Beclin-1 improves mitochondria-associated membranes in the heart during endotoxemia. FASEB Bioadv 3:123-135 (2021). PubMed: 33733054
  • Ye J  et al. Assessing the Magnitude of Immunogenic Cell Death Following Chemotherapy and Irradiation Reveals a New Strategy to Treat Pancreatic Cancer. Cancer Immunol Res 8:94-107 (2020). PubMed: 31719057
  • Nguyen QT  et al. Ufd1 phosphorylation at serine 229 negatively regulates endoplasmic reticulum-associated degradation by inhibiting the interaction of Ufd1 with VCP. Biochem J 476:2561-2577 (2019). PubMed: 31477623
  • Lee H  et al. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Nat Commun 7:12799 (2016). PubMed: 27619642

レビューと Q&A

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1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Calreticulin antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Armen Petrosyan

Verified customer

投稿 Oct 07 2021

Western blot abreview for Anti-Calreticulin antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Jul 01 2016

Western blot abreview for Anti-Calreticulin antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Jun 01 2016

Western blot abreview for Anti-Calreticulin antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Jul 23 2014

Immunohistochemistry (Frozen sections) abreview for Anti-Calreticulin antibody - ER Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Jun 03 2011

Question

I am asking the size of the ab4109 abcam (calreticulin-ER Marker, goat polyclonal to )
kindly reply
thank you

Read More
Answer

Thank you for contacting us.

The size of antibody as IgG is 150 kDa (25 kDa of each light chain and ˜53 kDa of each heavy chain).

The size of protein as mentioned before is 417 amino acid 48kDa.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

"may i know the molecular weight and size of the calreticulin antibody-ER Marker, ab4109. ty"

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Answer

Thank you for contacting us.

The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modifications at amino acid 48,159, 209 and 344.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Dear there
May i know the size i mean the molecular weight of the goat polyclonal to calreticulin-ER Marker ab4109 abcam. I checked it but am unable to find it. May you kindly let me know the size and molecular weight of the goat polyclonal to calreticulin-ER Marker ab4109 abcam
Thank you

Read More
Answer

Thank you for contacting us.

The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modification at amino acid 48,159, 209 and 344.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

I am looking for an Arabidopsis calreticulin antibody that
is suitable for Western blot
and/ or immunoprecipitation.
Internet search engines show an old entry for your
antibody ab2907,
but when I open your homepage, this reference is no longer
available.
Is there any Arabidopsis calreticulin antibody available?

Read More
Answer

Thank you for contacting us.

The antibody ab2907 was tried in Arabidopsis by a customer, and the results were submitted as an Abreview (2 out of 5 stars): https://www.abcam.com/Calreticulin-antibody-ER-Marker-ab2907.html&tab=abreviews&intabreviewid=24966

Thus, the Arabidopsis data is on the datasheet, but due to the rating of the results we do not list Arabidopsis as tested species or guarantee the antibody for it.

I have checked the homology of Arabidopsis with the human sequence and the homology is only 54% (isoform 1), 55% (isoform 2), and 49% (isoform 3) - thus very low. It is similar for the rabbit sequence: the homology between rabbit and Arabidopsis is also only 54%.
We usually suggest a sequence homology of 85% or higher forpotentialcross-reactivity. Thus, in generell it would be difficult to find an antibody raised against human or rabbit to be able to crossreact with Arabidopsis.

I might suggest to check http://www.biocompare.com for an antibody that was raised against an Arabidopsis sequence.

I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Hi, I need to know if this calreticulin 4109 will be good for elisa assays. I am doing an inhibition assay where I incubate human sera with the antiserum (polyclonal) against calreticulin and then search for binding of the antiserum to the calreticulin conatined in the serum. Please let me know if I can use your polyclonal antibody for that and also at what conc, would I have to use it for this type of assay?

Read More
Answer

This antibody has not been tested for use in ELISA assays. As it is supplied as a serum, it would not be suitable for use as a coating antibody. There is no immunological reason why it could not be used as a detection antibody, but we cannot guarantee its use in this application.

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