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  1. Link

    calreticulin-antibody-ab4109.pdf

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Tags & Cell Markers Subcellular Markers Organelles ER
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Anti-Calreticulin 抗体 (ab4109)

  • Datasheet
  • SDS
Reviews (4)Q&A (5)References (1)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Goat polyclonal to Calreticulin
  • Reacts with: Mouse, Rat, Rabbit, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Calreticulin protein (ab15729)
二次抗体
Product image
Donkey Anti-Goat IgG H&L (HRP) (ab97110)

関連製品

製品の概要

  • 製品名

    Anti-Calreticulin antibody
    Calreticulin 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to Calreticulin
  • 由来種

    Goat
  • 特異性

    Recognises calreticulin.
  • 種交差性

    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Purified rabbit skeletal muscle calreticulin

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Calreticulin protein (ab15729)
  • Related Products

    • Recombinant Calreticulin protein (ab15729)

アプリケーション

  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能

      Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
    • 配列類似性

      Belongs to the calreticulin family.
    • ドメイン

      Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
      The interaction with glycans occurs through a binding site in the globular lectin domain.
      The zinc binding sites are localized to the N-domain.
      Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
    • 細胞内局在

      Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
    • Target information above from: UniProt accession P27797 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 811 Human
      • Entrez Gene: 12317 Mouse
      • Entrez Gene: 100009050 Rabbit
      • Entrez Gene: 64202 Rat
      • Omim: 109091 Human
      • SwissProt: P27797 Human
      • SwissProt: P14211 Mouse
      • SwissProt: P15253 Rabbit
      • SwissProt: P18418 Rat
      • Unigene: 515162 Human
      • Unigene: 1971 Mouse
      • Unigene: 467043 Mouse
      • Unigene: 974 Rat
      see all
    • 別名

      • Autoantigen RO antibody
      • CALR antibody
      • CALR protein antibody
      • CALR_HUMAN antibody
      • Calregulin antibody
      • Calreticulin antibody
      • cC1qR antibody
      • CRP55 antibody
      • CRT antibody
      • CRTC antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • Epididymis secretory sperm binding protein Li 99n antibody
      • ERp60 antibody
      • FLJ26680 antibody
      • grp60 antibody
      • HACBP antibody
      • HEL S 99n antibody
      • RO antibody
      • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
      • Sicca syndrome antigen A antibody
      • SSA antibody
      see all

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (1)

    ab4109 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4109 は 1 報の論文で使用されています。

    • Lee H  et al. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Nat Commun 7:12799 (2016). PubMed: 27619642

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-Calreticulin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 01 2016

    Western blot abreview for Anti-Calreticulin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 01 2016

    Western blot abreview for Anti-Calreticulin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Rat Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2014

    Question

    I am asking the size of the ab4109 abcam (calreticulin-ER Marker, goat polyclonal to )
    kindly reply
    thank you

    Read More

    Abcam community

    Verified customer

    Asked on Jan 14 2013

    Answer

    Thank you for contacting us.

    The size of antibody as IgG is 150 kDa (25 kDa of each light chain and ˜53 kDa of each heavy chain).

    The size of protein as mentioned before is 417 amino acid 48kDa.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 14 2013

    Question

    "may i know the molecular weight and size of the calreticulin antibody-ER Marker, ab4109. ty"

    Read More

    Abcam community

    Verified customer

    Asked on Jan 11 2013

    Answer

    Thank you for contacting us.

    The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modifications at amino acid 48,159, 209 and 344.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 11 2013

    Question

    Dear there
    May i know the size i mean the molecular weight of the goat polyclonal to calreticulin-ER Marker ab4109 abcam. I checked it but am unable to find it. May you kindly let me know the size and molecular weight of the goat polyclonal to calreticulin-ER Marker ab4109 abcam
    Thank you

    Read More

    Abcam community

    Verified customer

    Asked on Jan 11 2013

    Answer

    Thank you for contacting us.

    The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modification at amino acid 48,159, 209 and 344.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 11 2013

    Question

    I am looking for an Arabidopsis calreticulin antibody that
    is suitable for Western blot
    and/ or immunoprecipitation.
    Internet search engines show an old entry for your
    antibody ab2907,
    but when I open your homepage, this reference is no longer
    available.
    Is there any Arabidopsis calreticulin antibody available?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 16 2012

    Answer

    Thank you for contacting us.

    The antibody ab2907 was tried in Arabidopsis by a customer, and the results were submitted as an Abreview (2 out of 5 stars): https://www.abcam.com/index.html?datasheet=2907&tab=abreviews&intabreviewid=24966

    Thus, the Arabidopsis data is on the datasheet, but due to the rating of the results we do not list Arabidopsis as tested species or guarantee the antibody for it.

    I have checked the homology of Arabidopsis with the human sequence and the homology is only 54% (isoform 1), 55% (isoform 2), and 49% (isoform 3) - thus very low. It is similar for the rabbit sequence: the homology between rabbit and Arabidopsis is also only 54%.
    We usually suggest a sequence homology of 85% or higher forpotentialcross-reactivity. Thus, in generell it would be difficult to find an antibody raised against human or rabbit to be able to crossreact with Arabidopsis.

    I might suggest to check http://www.biocompare.com for an antibody that was raised against an Arabidopsis sequence.

    I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 16 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Calreticulin antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 03 2011

    Question

    Hi, I need to know if this calreticulin 4109 will be good for elisa assays. I am doing an inhibition assay where I incubate human sera with the antiserum (polyclonal) against calreticulin and then search for binding of the antiserum to the calreticulin conatined in the serum. Please let me know if I can use your polyclonal antibody for that and also at what conc, would I have to use it for this type of assay?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 05 2001

    Answer

    This antibody has not been tested for use in ELISA assays. As it is supplied as a serum, it would not be suitable for use as a coating antibody. There is no immunological reason why it could not be used as a detection antibody, but we cannot guarantee its use in this application.

    Read More

    Abcam Scientific Support

    Answered on Feb 13 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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