保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: Proprietary, not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3, Protein carrier.
Concentration information loading...
Our Abpromise guarantee covers the use of ab700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/400. ABC system. 30mins at RT.
Calponin has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20618694
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20138661|
|IHC-P||1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC system. 30mins at RT.|
機能Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
組織特異性Smooth muscle, and tissues containing significant amounts of smooth muscle.
配列類似性Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
ab700 at a 1/50 dilution staining rat heart tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed prior to incubation with the antibody overnight at 4°C. An Alex-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary. The image was taken with a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode and shows stained Calponin (smooth muscle cells-green) and counterstained nuclei (DAPI-blue) (left panel).DIC (phase) image of the same tissue (right panel) confirm the localization of the calponin staining in the arterial smooth muscle in the heart tissue with very little background.
ab700 staining Calponin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).
This product has been referenced in:
- Malashicheva A et al. Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms. Int J Vasc Med 2016:3107879 (2016). ICC . Read more (PubMed: 26904289) »
- Yuan K et al. Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis. Am J Pathol 185:69-84 (2015). Read more (PubMed: 25447046) »