Application
Western blot
Sample
Cow Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Dako goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
Positive control
Lane 1: bovine kidney
Lane 2: bovine kidney
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 22 2016