Anti-Calponin 1 抗体 [CNN1/832 + CALP] (ab233854)
Key features and details
- Mouse monoclonal [CNN1/832 + CALP] to Calponin 1
- Suitable for: ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Calponin 1 antibody [CNN1/832 + CALP]
Calponin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CNN1/832 + CALP] to Calponin 1 -
由来種
Mouse -
アプリケーション
適用あり: ICC, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Human Calponin 1 aa 1 to the C-terminus. (CNN1/832).
Database link: P51911
Tissue, cells or virus. (Crude human uterus extract (CALP)). -
ポジティブ・コントロール
- IHC-P: Human uterus and breast carcinoma tissues; Rat uterus tissues. ICC: K562 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from Bioreactor Concentrate by Protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
CNN1/832 + CALP -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233854の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
特記事項 |
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ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
ターゲット情報
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機能
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
組織特異性
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
配列類似性
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1264 Human
- Entrez Gene: 65204 Rat
- Omim: 600806 Human
- SwissProt: P51911 Human
- SwissProt: Q08290 Rat
- Unigene: 465929 Human
- Unigene: 31788 Rat
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別名
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
画像
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Formalin-fixed, paraffin-embedded human uterus tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
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Immunocytochemistry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Calponin 1 with ab233854 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded rat uterus tissue stained for Calponin 1 using ab233854 at 1 µg/ml in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab233854 は 1 報の論文で使用されています。
- Huang CW et al. Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerve. Stem Cell Res Ther 10:234 (2019). PubMed: 31376835