Anti-Calponin 1 抗体 [CALP] (ab700)

Reviews (3)Q&A (2)References (20)

Key features and details

  • Mouse monoclonal [CALP] to Calponin 1
  • Reacts with: Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Calponin 1 antibody [CALP]
    Calponin 1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CALP] to Calponin 1

  • 由来種

    Mouse

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Other Immunogen Type corresponding to Human Calponin 1.

  • ポジティブ･コントロール

    • Myoepithelium in normal breast glands.

製品の特性

ターゲット情報

  • 機能

    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

  • 組織特異性

    Smooth muscle, and tissues containing significant amounts of smooth muscle.

  • 配列類似性

    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Basic calponin antibody
      • Calponin 1 antibody
      • Calponin 1 basic smooth muscle antibody
      • Calponin H1 antibody
      • Calponin H1 smooth muscle antibody
      • Calponin-1 antibody
      • Calponin1 antibody
      • Calponins basic antibody
      • CNN 1 antibody
      • Cnn1 antibody
      • CNN1_HUMAN antibody
      • Epididymis secretory protein Li 14 antibody
      • HEL S 14 antibody
      • Sm Calp antibody
      • SMCC antibody
      • smooth muscle antibody
      see all

    参考文献 (20)

    ab700 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab700 は 20 報の論文で使用されています。

    • Fang L  et al. Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells via interaction with tropoelastin mRNA. Int J Mol Med 43:1597-1610 (2019). PubMed: 30720050
    • Kasprzyk-Pawelec A  et al. microRNA expression profile in Smooth Muscle Cells isolated from thoracic aortic aneurysm samples. Adv Med Sci 64:331-337 (2019). PubMed: 31022558
    • Maclellan RA  et al. Analysis of Follicle-Stimulating Hormone Receptor in Infantile Hemangioma. Ann Plast Surg 80:S211-S213 (2018). PubMed: 29596087
    • Tamosiuniene R  et al. Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension. Circ Res 122:1689-1702 (2018). PubMed: 29545367
    • Ventura Ferreira MS  et al. Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta. Stem Cell Res Ther 9:28 (2018). PubMed: 29402304
    View all Publications for this product

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calponin 1 antibody [CALP]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human arterial myocytes)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Human arterial myocytes
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 08 2015

    Question

    We need an anti-calponin antibody with proven specificity that we can use to assess calponin expression in rat cells. Your company noted that CALP (ab700) works for flow cytometry in human cells but there is no immunoblotting data to indicate that it recognizes a single band. Do you have specificity data for this antibody that you can share with us?

    Read More
    Answer

    The first reference on the datasheet contains the only western blot I have found for this clone, in Figure 8, but it is cropped.

    Wu W et al. Artificial niche combining elastomeric substrate and platelets guides vascular differentiation of bone marrow mononuclear cells. Tissue Eng Part A 17:1979-92 (2011).

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142637/

    The earliest references we have for this clone, from 1999, appear to characterize the antibody only by IHC. I do not have access to those journals to confirm.

    Have you considered the rabbit monoclonal ab46794? The western blot suggests good specificity but as far as we know, it has never been tested in flow cytometry, only ICC and a few other applications.

    https://www.abcam.com/index.html?datasheet=700

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Calponin 1 antibody [CALP]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Smooth muscle cells from mesenteric artery)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Smooth muscle cells from mesenteric artery
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 17 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calponin 1 antibody [CALP]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Heart tissue)
    Antigen retrieval step
    Heat mediated
    Specification
    Heart tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
    Fixative
    Formaldehyde
    Read More

    Dr. Mal Niladri

    Verified customer

    投稿 Oct 16 2006

    Question

    I wish to buy antibodies that can detect human Calponin 1. You catalog list a calponin monoclonal antibody but does not state whether it is caplonin 1,2, or 3. Could you please advise.

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    Answer

    As far as we are aware, this antibody detects all three.

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