Anti-Calpain small subunit 1 抗体 [EPR3323] (ab92356)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3323] to Calpain small subunit 1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Calpain small subunit 1 antibody [EPR3323]
Calpain small subunit 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3323] to Calpain small subunit 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human Calpain small subunit 1 aa 50-150. The exact sequence is proprietary.
Database link: P04632 -
ポジティブ・コントロール
- WB: Hap1 cells, HepG2 cells, HeLa cells; T47-D cell lysate, HEK293T cell lysate, A431 cell lysate, Human spleen lysate. IHC-P: human kidney.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3323 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92356の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized antibody is recommended as these have been shown to give superior staining. |
特記事項 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended as these have been shown to give superior staining. |
ターゲット情報
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機能
Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
配列類似性
Contains 5 EF-hand domains. -
ドメイン
The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant. -
細胞内局在
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding. - Information by UniProt
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参照データベース
- Entrez Gene: 826 Human
- Entrez Gene: 12336 Mouse
- Entrez Gene: 29156 Rat
- Omim: 114170 Human
- SwissProt: P04632 Human
- SwissProt: O88456 Mouse
- SwissProt: Q64537 Rat
- Unigene: 515371 Human
see all -
別名
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
画像
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All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CAPNS1 (Calpain small subunit 1) knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 28 kDaLanes 1 - 4: Merged signal (red and green). Green - ab92356 observed at 82 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92356 was shown to specifically react with Calpain 1 in wild-type HAP1 cells as signal was lost in CAPNS1 (Calpain small subunit 1) knockout cells. Wild-type and CAPNS1 (Calpain small subunit 1) knockout samples were subjected to SDS-PAGE. ab92356 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Calpain small subunit 1 antibody [EPR3323] (ab92356) at 1/1000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 28 kDa -
ab92356 at 1/100 dilution staining Calpain small subunit 1 in paraffin-embedded Human kidney
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab92356 は 4 報の論文で使用されています。
- Lin X et al. Circular RNA ABCB10 promotes cell proliferation and invasion, but inhibits apoptosis via regulating the microRNA-1271-mediated Capn4/Wnt/ß-catenin signaling pathway in epithelial ovarian cancer. Mol Med Rep 23:N/A (2021). PubMed: 33760208
- Ding H et al. Extracellular vesicles derived from bone marrow mesenchymal stem cells alleviate neuroinflammation after diabetic intracerebral hemorrhage via the miR-183-5p/PDCD4/NLRP3 pathway. J Endocrinol Invest N/A:N/A (2021). PubMed: 34024028
- Seymen CM et al. Melatonin Modulates NMDA-Receptor 2B/Calpain-1/Caspase-12 Pathways in Rat Brain After Long Time Exposure to GSM Radiation. Turk Neurosurg N/A:N/A (2019). WB, IHC-P ; Rat . PubMed: 31608966
- Kim DH et al. Calpain 9 as a therapeutic target in TGFß-induced mesenchymal transition and fibrosis. Sci Transl Med 11:N/A (2019). PubMed: 31316008