Anti-Calnexin 抗体 [EPR21205] (ab213243)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21205] to Calnexin
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
製品の概要
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製品名
Anti-Calnexin antibody [EPR21205]
Calnexin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21205] to Calnexin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein (His-tag) corresponding to Mouse Calnexin. Expressed in HEK293 Cells
Database link: NP_031623.1 -
ポジティブ・コントロール
- WB: NIH3T3, HeLa, HAP1
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特記事項
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
EPR21205 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
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Matched antibody pairs
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab213243の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 68 kDa.
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特記事項 |
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WB
Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 68 kDa. |
ターゲット情報
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機能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
配列類似性
Belongs to the calreticulin family. -
細胞内局在
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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別名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
画像
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Lane 1: NIH3T3 whole cell lysate (10 µg)
Lane 2: Mouse brain whole tissue lysate (10 µg)
Lane 3: HeLa whole cell lysate (10 µg)
Lane 4: Hap1 Wild-type whole cell lysate (10 µg)
Lane 5: CANX knockout Hap1 whole cell lysate (10 µg)Lanes 1 - 5: Merged signal (red and green). Green - ab213243 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab213243 was shown to specifically react with Calnexin in wild-type HAP1 cells as signal was lost in Calnexin (CANX) knockout cells. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Nitrocellulose membranes were blocked in 3% milk in TBST before ab213243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1µg/mL and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab213243 は 6 報の論文で使用されています。
- Pinson MR et al. Gag-like proteins: Novel mediators of prenatal alcohol exposure in neural development. Alcohol Clin Exp Res 46:556-569 (2022). PubMed: 35187673
- Kumar P et al. Tumour-associated antigenic peptides are present in the HLA class I ligandome of cancer cell line derived extracellular vesicles. Immunology 166:249-264 (2022). PubMed: 35318648
- Liu Z et al. Exosome-mediated transduction of mechanical force regulates prostate cancer migration via microRNA. Biochem Biophys Rep 31:101299 (2022). PubMed: 35812347
- Wang X et al. Exosome-delivered circRNA promotes glycolysis to induce chemoresistance through the miR-122-PKM2 axis in colorectal cancer. Mol Oncol 14:539-555 (2020). PubMed: 31901148
- Xue J et al. Addition of High Molecular Weight Hyaluronic Acid to Fibroblast-Like Stromal Cells Modulates Endogenous Hyaluronic Acid Metabolism and Enhances Proteolytic Processing and Secretion of Versican. Cells 9:N/A (2020). PubMed: 32668663
- Fan SJ et al. Glutamine deprivation alters the origin and function of cancer cell exosomes. EMBO J 39:e103009 (2020). PubMed: 32720716