Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5028] to Calmodulin 1/2/3 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-Calmodulin 1/2/3 antibody [EPR5028] - BSA and Azide free
Calmodulin 1/2/3 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5028] to Calmodulin 1/2/3 - BSA and Azide free
アプリケーション適用あり: WB, Flow Cyt, IPmore details
適用なし: ICC or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Calmodulin 1/2/3 aa 1-100 (N terminal). The exact sequence is proprietary.
Ab247990 is the carrier-free version of ab124742. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247990 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
関連性Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
細胞内局在Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
製品の状態There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
All lanes : Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
This data was developed using ab124742, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.