Calcium Assay Kit (Colorimetric) (ab102505)
製品の概要
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製品名
Calcium Assay Kit (Colorimetric)
Calcium キット 製品一覧 -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
アッセイタイプ
Quantitative -
検出感度
> 0.1 mM -
検出範囲
0.1 mM - 25 mM -
全工程の試験時間
0h 20m -
種交差性
交差種: Other species, Mammals -
製品の概要
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM).
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
This calcium assay detects free calcium ions only.
Calcium assay protocol summary:
- add samples and standards to wells
- add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- analyze with microplate reader -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 250 tests Calcium Assay Buffer NM 1 x 15ml Calcium Standard Yellow 1 x 100µl Chromogenic Reagent NM 1 x 25ml -
研究分野
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関連性
Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. -
別名
- Ca++
- Ca2+
関連製品
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Corresponding kit
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Related Products
画像
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Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)G?r?gh, Tibor et al., BMC musculoskeletal disorders?vol. 18,1 500., Fig 3, doi:10.1186/s12891-017-1860-2
Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate
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Functional Studies - Calcium Assay Kit (Colorimetric) (ab102505)Koskela, A et al., Scientific reports?vol. 7,1 6841., Fig 4, doi:10.1038/s41598-017-07359-6Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
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Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.
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Example of standard curve obtained using ab102505
プロトコール
参考文献
This product has been referenced in:
- Zhou YD & Cai L Calpeptin Reduces Neurobehavioral Deficits and Neuronal Apoptosis Following Subarachnoid Hemorrhage in Rats. J Stroke Cerebrovasc Dis 28:125-132 (2019). Read more (PubMed: 30337208) »
- Yi J et al. Effects of continuous and intermittent parathyroid hormone administration on midpalatal suture expansion in rats. Arch Oral Biol 99:161-168 (2019). Read more (PubMed: 30710837) »