Anti-Calcitonin 抗体 (ab8553)
Key features and details
- Rabbit polyclonal to Calcitonin
- Suitable for: Sandwich ELISA, ELISA, IHC-FoFr, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Calcitonin antibody
Calcitonin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcitonin -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, ELISA, IHC-FoFr, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Horse, Dog, Pig
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免疫原
Synthetic peptide within Human Calcitonin aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P01258 -
ポジティブ･コントロール
- Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Prolonged fixation in buffered formalin can destroy the epitope
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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sELISA pair antibody
アプリケーション
Our Abpromise guarantee covers the use of ab8553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab11493 as Capture. |
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| ELISA | 1/300 - 1/3000. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-P | 1/300. Antigen retrieval is not essential but may optimise staining. | |
| WB | 1/200 - 1/500. Predicted molecular weight: 15 kDa. |
ターゲット情報
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関連性
Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity. -
細胞内局在
Cytoplasmic and Secreted -
参照データベース
- Entrez Gene: 403946 Dog
- Entrez Gene: 796 Human
- Entrez Gene: 12310 Mouse
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- SwissProt: P41547 Dog
- SwissProt: P01258 Human
- SwissProt: P70160 Mouse
see all -
別名
- Alpha CGRP antibody
- alpha type CGRP antibody
- CALC 1 antibody
see all
画像
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Sandwich ELISA - Anti-Calcitonin antibody (ab8553)Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5 ] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml -
Western blot - Anti-Calcitonin antibody (ab8553)Anti-Calcitonin antibody (ab8553) at 1/25 dilution + Human thyroid lysate.
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody (ab8553)Immunohistochemical analysis of human thyroid staining with ab8553.
プロトコール
参考文献 (1)
ab8553 は 1 報の論文で使用されています。
- Yerbury JJ et al. alpha2-Macroglobulin and haptoglobin suppress amyloid formation by interacting with prefibrillar protein species. J Biol Chem 284:4246-54 (2009). WB ; Human . PubMed: 19074141
