Anti-Calcineurin inhibitor 抗体 (ab3349)

Reviews (2)Q&A (3)References (9)
Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)

Key features and details

  • Rabbit polyclonal to Calcineurin inhibitor
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Calcineurin inhibitor antibody

  • 製品の詳細

    Rabbit polyclonal to Calcineurin inhibitor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Synthetic peptide (His-tag) corresponding to Rat Calcineurin inhibitor aa 1850 to the C-terminus.
    Database link: O88480

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • ICC/IF: PC12 and NRK cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituent: 99% PBS
  • Concentration information loading...

  • 精製度

    Whole antiserum

  • 一次抗体 備考

    Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3349の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/1000.
特記事項
ICC/IF
1/1000.

ターゲット情報

  • 機能

    May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals.

  • 組織特異性

    Widely expressed in different tissues.

  • 配列類似性

    Contains 6 TPR repeats.

  • 翻訳後修飾

    Activated through PKC-mediated hyperphosphorylation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A330070M20Rik antibody
      • Cabin 1 antibody
      • CABIN_HUMAN antibody
      • Cabin1 antibody
      • CAIN antibody
      • Calcineurin binding protein cabin 1 antibody
      • Calcineurin inhibitor antibody
      • Calcineurin-binding protein cabin-1 antibody
      • KIAA0330 antibody
      • PPP3IN antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)
      Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)
      Immunocytochemistry/ Immunofluorescence analysis of NRK cells labeling Calcineurin inhibitor with ab3349. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with ab3349 at a dilution of 20 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1/1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye.
    • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)
      Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)

      ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献 (9)

    ab3349 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3349 は 9 報の論文で使用されています。

    • Smith R  et al. HIRA contributes to zygote formation in mice and is implicated in human 1PN zygote phenotype. Reproduction 161:697-707 (2021). PubMed: 33835048
    • Hoang SM  et al. Regulation of ALT-associated homology-directed repair by polyADP-ribosylation. Nat Struct Mol Biol 27:1152-1164 (2020). PubMed: 33046907
    • Torné J  et al. Two HIRA-dependent pathways mediate H3.3 de novo deposition and recycling during transcription. Nat Struct Mol Biol 27:1057-1068 (2020). PubMed: 32895554
    • Zhang L  et al. Downregulation of miRNA-15a and miRNA-16 promote tumor proliferation in multiple myeloma by increasing CABIN1 expression. Oncol Lett 15:1287-1296 (2018). PubMed: 29399181
    • Ray-Gallet D  et al. Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit. Nat Commun 9:3103 (2018). PubMed: 30082790
    • Lee JS & Zhang Z O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence. Proc Natl Acad Sci U S A 113:E3213-20 (2016). PubMed: 27217568
    • Ying CY  et al. MEF2B mutations lead to deregulated expression of the oncogene BCL6 in diffuse large B cell lymphoma. Nat Immunol 14:1084-92 (2013). PubMed: 23974956
    • Rai TS  et al. Human CABIN1 is a functional member of the human HIRA/UBN1/ASF1a histone H3.3 chaperone complex. Mol Cell Biol 31:4107-18 (2011). WB, ICC/IF, IP ; Human . PubMed: 21807893
    • Elsaesser SJ & Allis CD HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Cold Spring Harb Symp Quant Biol 75:27-34 (2010). WB . PubMed: 21047901

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Calcineurin inhibitor antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (heart)
    Permeabilization
    Yes - 0.3% Triton X
    Specification
    heart
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 11 2015

    Western blot abreview for Anti-Calcineurin inhibitor antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole Embryo)
    Gel Running Conditions
    Reduced Denaturing (4%-20%)
    Specification
    Whole Embryo
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 13 2013

    Question

    I would like to test ab3349 in mouse.

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    Answer


    I am very pleased to hear you would like to accept our offer and test ab3349 in mouse. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Question

    Phone call requesting concentration of antibody ab3349 (lot number 701258)

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    Answer

    Thank you for contacting us. As discussed on the phone this antibody stock is whole serum it is therefore difficult to determine the concentration of the antibody, since whole serum contains many other host serum proteins besides the antibody of interest. The amount of anti-CAIN IgG in the antiserum for ab3349 has never been determined. In general terms rabbit serum such as this generally contains 1 to 10 mg/ml of specific IgG. For every application used, optimisation of the antibody dilution will need to be performed but as I starting point I would suggest the following: WB: 1/500 IHC/ICC: 1/50-1/100 ELISA: 1/500 FACS/flow cytometry: 1/500 IP: 1/50-1/100 Unfortunately I do not have any more specific information. If you have any further questions please do not heistate to ask.

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    Question

    I would like to test a few of your antibodies as part of the recent testing offer promotion. In the first instance, I'd like to trial ab57137 - GLTSCR2 antibody. I'd like to trial this antibody in canine and human cells, for both immunofluorescence and co-immunoprecipitation experiments. I look forward to hearing from you. Kind regards,

    Read More
    Answer

    Thank you for contacting us. We are sorry; ab57137 is no longer included in our product range. We apologize for the inconvenience caused. Could we recommend few other products from our catalogue? We have ab3349 and ab76600 antibodies available to buy. ab76600; the discount code can be provided for IP, ICC/IF or Dog species. ab3349; the discount code can be provided for Dog species only. We also have peptide ab88931 that can be used in studies with ab76600 as blocking peptide. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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