Anti-Calcineurin B/PPP3R1 抗体 (ab230899)
Key features and details
- Rabbit polyclonal to Calcineurin B/PPP3R1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Calcineurin B/PPP3R1 antibody
Calcineurin B/PPP3R1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcineurin B/PPP3R1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow -
免疫原
Recombinant fragment corresponding to Human Calcineurin B/PPP3R1 aa 1 to the C-terminus.
Database link: P63098 -
ポジティブ・コントロール
- WB: SH-SY5Y, SW480, HL-60 and MCF7 cell extracts; mouse brain, spleen and lung cell extracts; rat brain and testis cell extracts. IHC-P: Human brain cortex tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- MCF7 whole cell lysate (ab29537)
- Mouse brain tissue lysate - total protein (ab30151)
- SW480 whole cell lysate (ab3957)
- Mouse brain tissue lysate - total protein (ab4022)
- HL-60 whole cell lysate (ab7914)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab230899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/2000. Predicted molecular weight: 19 kDa.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/2000. Predicted molecular weight: 19 kDa. |
ターゲット情報
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機能
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. -
配列類似性
Belongs to the calcineurin regulatory subunit family.
Contains 4 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 282321 Cow
- Entrez Gene: 5534 Human
- Entrez Gene: 19058 Mouse
- Entrez Gene: 29748 Rat
- Omim: 601302 Human
- SwissProt: P63099 Cow
- SwissProt: P63098 Human
- SwissProt: Q63810 Mouse
see all -
別名
- alpha isoform (calcineurin B, type I) antibody
- calcineurin B, type I (19kDa) antibody
- Calcineurin subunit B type 1 antibody
see all
画像
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All lanes : Anti-Calcineurin B/PPP3R1 antibody (ab230899) at 1/500 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell extract
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) cell extract
Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 5 : Mouse brain cell extract
Lane 6 : Mouse spleen cell extract
Lane 7 : Mouse lung cell extract
Lane 8 : Rat brain cell extract
Lane 9 : Rat testis cell extract
Developed using the ECL technique.
Predicted band size: 19 kDa -
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Calcineurin B/PPP3R1 with ab230899 at 1/50 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab230899 は論文での使用が確認できていません。