Anti-Calcineurin B/PPP3R1 抗体 (ab154650)
Key features and details
- Rabbit polyclonal to Calcineurin B/PPP3R1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Calcineurin B/PPP3R1 antibody
Calcineurin B/PPP3R1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcineurin B/PPP3R1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
免疫原
Recombinant fragment corresponding to Human Calcineurin B/PPP3R1 aa 1-150.
Database link: P63098 -
ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, Molt 4 and Raji cell lysates; Human NCI-N87 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154650の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 19 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 19 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ターゲット情報
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機能
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. -
配列類似性
Belongs to the calcineurin regulatory subunit family.
Contains 4 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 378804 Chicken
- Entrez Gene: 282321 Cow
- Entrez Gene: 5534 Human
- Entrez Gene: 19058 Mouse
- Entrez Gene: 29748 Rat
- Entrez Gene: 399215 Xenopus laevis
- Entrez Gene: 395037 Xenopus tropicalis
- Omim: 601302 Human
see all -
別名
- alpha isoform (calcineurin B, type I) antibody
- calcineurin B, type I (19kDa) antibody
- Calcineurin subunit B type 1 antibody
see all
画像
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All lanes : Anti-Calcineurin B/PPP3R1 antibody (ab154650) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : Hep G2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa
15% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling Calcineurin B/PPP3R1 with ab154650 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab154650 は 5 報の論文で使用されています。
- Shoda W et al. Sodium-calcium exchanger 1 is the key molecule for urinary potassium excretion against acute hyperkalemia. PLoS One 15:e0235360 (2020). PubMed: 32603346
- Marciel MP et al. Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis. Oncotarget 9:13407-13422 (2018). PubMed: 29568366
- Olivares-Florez S et al. Nuclear calcineurin is a sensor for detecting Ca2+ release from the nuclear envelope via IP3R. J Mol Med (Berl) 96:1239-1249 (2018). PubMed: 30293136
- Shi X et al. Quality-control method for the determination of biological activity of engineered calcineurin subunit B. Sci China Life Sci 59:584-8 (2016). PubMed: 27053126
- Zhu X et al. Cardiac-Specific EPI64C Blunts Pressure Overload-Induced Cardiac Hypertrophy. Hypertension 67:866-77 (2016). PubMed: 27021007