Anti-Calcineurin A 抗体 (ab3673)

Reviews (5)Q&A (6)References (19)
Western blot - Anti-Calcineurin A antibody (ab3673)
  • Western blot - Anti-Calcineurin A antibody (ab3673)
  • Western blot - Anti-Calcineurin A antibody (ab3673)
  • Western blot - Anti-Calcineurin A antibody (ab3673)

Key features and details

  • Rabbit polyclonal to Calcineurin A
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Calcineurin A antibody
    Calcineurin A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Calcineurin A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human, Recombinant fragment

  • 免疫原

    Recombinant full length protein corresponding to Human Calcineurin A.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3673の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
1/2000 - 1/5000. Detects a band of approximately 62 kDa.
特記事項
WB
1/2000 - 1/5000. Detects a band of approximately 62 kDa.

ターゲット情報

  • 機能

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

  • 配列類似性

    Belongs to the PPP phosphatase family. PP-2B subfamily.

  • 細胞内局在

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

画像

  • Western blot - Anti-Calcineurin A antibody (ab3673)
    Western blot - Anti-Calcineurin A antibody (ab3673)
    All lanes : Anti-Calcineurin A antibody (ab3673) at 1/200 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 62 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Calcineurin A antibody (ab3673)
    Western blot - Anti-Calcineurin A antibody (ab3673)

    Western Blot of wild type mouse brain tissue homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).

  • Western blot - Anti-Calcineurin A antibody (ab3673)
    Western blot - Anti-Calcineurin A antibody (ab3673)This image is a courtesy of Anonymous Abreview
    Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Huh7 (Human hepatocellular carcinoma) whole cell lysate at 15000 cells

    Secondary
    HRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilution

    Performed under reducing conditions.

    Observed band size: 62 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-Calcineurin A antibody (ab3673)
    Western blot - Anti-Calcineurin A antibody (ab3673)
    Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Recombinant Human Calcineurin A protein (ab101870) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

参考文献 (19)

ab3673 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3673 は 19 報の論文で使用されています。

  • Bella P  et al. Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy. EMBO Mol Med 12:e11019 (2020). PubMed: 31793167
  • Sánchez-Solano A  et al. Regulation of the Ca2+-activated chloride channel Anoctamin-1 (TMEM16A) by Ca2+-induced interaction with FKBP12 and calcineurin. Cell Calcium 89:102211 (2020). PubMed: 32422433
  • Zheng J  et al. Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. Eur J Pharmacol 883:173386 (2020). PubMed: 32712088
  • Chen X  et al. Adropin regulates hepatic glucose production via PP2A/AMPK pathway in insulin-resistant hepatocytes. FASEB J 34:10056-10072 (2020). PubMed: 32579277
  • Xu X  et al. Calcium inhibitor inhibits high glucose-induced hypertrophy of H9C2 cells. Mol Med Rep 22:1783-1792 (2020). PubMed: 32705176
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-Calcineurin A antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 May 29 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Calcineurin A antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Dissociated hippocampal cells)
Specification
Dissociated hippocampal cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton 5min RT
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Read More

Dr. Matthew Grubb

Verified customer

投稿 Oct 24 2012

Western blot abreview for Anti-Calcineurin A antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
20 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Jan 05 2010

Immunoprecipitation abreview for Anti-Calcineurin A antibody

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (vero)
Total protein in input
1e+006 cells
Specification
vero
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

投稿 Nov 24 2009

Western blot abreview for Anti-Calcineurin A antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cells)
Specification
huh-7 cells
Treatment
viral protein expressed
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 minute(s) · Concentration: 0.5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Jul 08 2009

Question

Dear Sir/Madam
As per my enquiry below, would we qualify for the testing discount program on this product?
Yours

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Answer

Thank you for your interest.
Would you be so kind to provide a summary of the project (sample type, species, application, controls etc) you wish to use this antibody?
I would also advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/collaborationdiscount.
I look forward to hearing from you soon.

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Question

Please see below for the response from the customer;

Thanks so much. Thanks for your explanation and we very much will appreciate trying a new lot number of the antibody on our samples.

Thanks again. I will let you know if we are successful in detecting the cleavage product.
If you could please send a vial from a different lot that would be great J

Thanks for your help and hope to hear from you soon

Kind regards,

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as expected and for the inconvenience this has caused. As requested, I have issued a free of charge replacement from a different lot with the number FOCR PO-15281 in Order 1206011

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

Hi Abcam,

Please see below for an email we have received from a customer;

I am writing with regards to your anti-Calcineurin antibody a3673 and the Abcam promise guarnatee.
This antibody has been used in our lab for many years at a concentration of 1:2000 in Western Blots to detect two bands of interest: The 61 kDa band, and the 48kDa cleavage product. We load 100ug of total protein and during the chemiluminescence exposure, we overexpose the more abundant 61 kDa band, to reveal the 48kDa claeavage product we are interested in.
In a comparison experiment, two seperate gels, loaded with identical samples of mouse brain homogenate, were transfered to two blots. One was probed with the old (effective) anti-Calcineurin antibody a3673, one with a new one purchased in August (lot number GR89521-1, 100ul).( PO 47096590 )
The new antibody completely failed to detect the presence of the 48kDa band (see attached scan). As this is the band we are interested in, and purchased the antibody only because it was in the past binding to this band, we would like to enquire about a replacement (a different lot number) or an alternative product you could offer us as part of your guarantee.
I look forward to your reply,

Thanks for your help and hope to hear from you soon

Kind regards,

Read More
Answer

Thank you for contacting us.

I can confirm that the Anti-Calcineurin A antibody (ab3673) is a ployclonal antibody. The affinity of these types of antibodies will change with different rounds of immunisation as well as different hosts. With different host even the epitopes of recognition might change. For that reason it is not unusual that with another lot the detection of the cleavage product might change.

We would be pleased to make an exception in this special case and offer you another lot of this product to try it with your samples. Please note that we cannot guarantee that this lot will detect the cleavage product the same as the first lot you purchased.

I am sorry that I can not be of more help then that on this occasion. I look forward to hearing from you with details of how you would like to proceed.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

You're right, this will be immunocytochemistry - apologies for the error.
The cells are rat hippocampal neurons, dissected at E18, dissociated in trypsin, then grown for ˜10d on laminin- and PDL-coated coverslips, then fixed in paraformaldehyde (normally 4% for 20min, but we may try weaker fixation regimes to try and improve staining if necessary).
Yours

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Answer

DISCOUNT CODE: 100%ABR-EXXXX
Expiration date: 25/01/2013
I am very pleased to hear you would like to accept our offer and test ab3673 in ICC in rat hippocampal cells.
This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ICC in rat hippocampal cells and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.
Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Dear Sir/Madam
We'll be using this antibody for immunohistochemistry in dissociated hippocampal neurons. We have a great deal of experience in immuno labelling in these cells, and we know that particular treatments applied to the neurons should change the intensity and/or distribution of labelling for calcineurin. We also have numerous controls to either overexpress or block calcineurin in these neurons, so if we see staining changes in treatment groups we can be very sure of their authenticity.
We're also happy to accept the terms and confitions of the 100% testing discount initiative
I look forward to hearing back from you with a discount code
Yours sincerely

Read More
Answer

Thank you for your response.
Could you please confirm if the cells are human or murine origin?
As I understand you wish to test this antibody in immunocytochemistry and not immunohistochemistry. What method will be used to dissociate the hippocampal neurons and for fixating them? Enzymatic or other techniques?
It is important for us and for our customer to distinguish between these two applications Immunocytochemistry (ICC) and immunohistochemistry (IHC-P or IHC-Fr).
I look forward to hearing from you soon.

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1-10 of 11 Abreviews or Q&A

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