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Anti-Cadherin 16 抗体 [CDH16/1532R] - BSA and Azide free (ab270294)

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Western blot - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
  • Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
  • Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CDH16/1532R] to Cadherin 16 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free
    Cadherin 16 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [CDH16/1532R] to Cadherin 16 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant full length protein corresponding to Human Cadherin 16.
    Database link: O75309

  • ポジティブ･コントロール

    • IHC-P: Human renal cell carcinoma tissue, rat kidney tissue and mouse kidney tissue. WB: Human kidney lysate. Flow Cyt: HEK-293 cells.

  • 特記事項

    ab270294 is a carrier free version of ab270263.

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    CDH16/1532R

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IHC-P
Mouse
Rat
Human
WB
Human
アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 90 kDa).
特記事項
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 90 kDa).

ターゲット情報

  • 機能

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

  • 組織特異性

    Kidney specific.

  • 配列類似性

    Contains 6 cadherin domains.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CAD16_HUMAN antibody
    • Cadherin 16 antibody
    • Cadherin 16 KSP cadherin antibody
    • Cadherin kidney antibody
    • Cadherin-16 antibody
    • CDH 16 antibody
    • CDH16 antibody
    • Kidney cadherin antibody
    • Kidney specific cadherin antibody
    • Kidney-specific cadherin antibody
    • Ksp cadherin antibody
    • Ksp-cadherin antibody
    • PRO1340 antibody
    • UNQ695 antibody
    see all

画像

  • Western blot - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Western blot - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Anti-Cadherin 16 antibody [CDH16/1532R] (ab270263) at 2 µg/ml + Human kidney lysate

    Predicted band size: 90 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)

    Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)

    Formalin-fixed, paraffin-embedded rat kidney tissue stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

  • Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)

    Flow cytometric analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Cadherin 16 (Blue) using ab270263 at 2µg/106 cells followed by goat anti-rabbit IgG-CF488; isotype control (Red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

  • Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)
    Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (ab270294)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab270294 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab270294 は論文での使用が確認できていません。

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