Anti-CACNA1A 抗体 (ab32642)
Key features and details
- Rabbit polyclonal to CACNA1A
- Suitable for: WB, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CACNA1A antibody -
製品の詳細
Rabbit polyclonal to CACNA1A -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, IHC-FoFrmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Cow -
免疫原
Synthetic peptide corresponding to Rat CACNA1A aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32641) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32642の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 282 kDa.
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IHC-FoFr | (1) |
1/100.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 282 kDa. |
IHC-FoFr
1/100. |
ターゲット情報
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関連性
Cav2.1 is a voltage-sensitive calcium channels (VSCC) which belongs to the calcium channel alpha-1 subunit family. Cav2.1 mediates the entry of calcium ions into excitable cells and is also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Cav2.1 (isoform alpha-1A) gives rise to P and/or Q-type calcium currents. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 282648 Cow
- Entrez Gene: 773 Human
- Entrez Gene: 12286 Mouse
- Entrez Gene: 100009265 Rabbit
- Entrez Gene: 25398 Rat
- Omim: 601011 Human
- SwissProt: O00555 Human
- SwissProt: P97445 Mouse
see all -
別名
- APCA antibody
- BI antibody
- Brain calcium channel 1 antibody
see all
画像
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All lanes : Anti-CACNA1A antibody (ab32642) at 1 µg/ml
Lane 1 : E16 Rat Embryo Brain - Tissue Lysate
Lane 2 : E16 Mouse Embryo Brain - Tissue Lysate
Lane 3 : E18 Rat Embryo Brain - Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 282 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32642 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Anti-CACNA1A antibody (ab32642) at 1 µg/ml + Cerebellum Rat Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 282 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunofluorescent staining for CACNA1A using Rabbit polyclonal to CACNA1A (ab32642)in rat Purkinje (arrows) and granular (arrow heads) cells, of the rat cerebellum. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32642 was incubated overnight at 1/3000 at room temperature. Secondary antibody Alexa fluor 488 1/3000 was incubated for 2 hours at room temperature.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab32642 は 3 報の論文で使用されています。
- Corona C et al. Activating Transcription Factor 4 (ATF4) Regulates Neuronal Activity by Controlling GABABR Trafficking. J Neurosci 38:6102-6113 (2018). PubMed: 29875265
- Grauel MK et al. RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses. Proc Natl Acad Sci U S A 113:11615-11620 (2016). PubMed: 27671655
- Vares G et al. Gene silencing of Tead3 abrogates radiation-induced adaptive response in cultured mouse limb bud cells. J Radiat Res (Tokyo) 52:39-46 (2011). WB ; Mouse . PubMed: 21293071