リコンビナントRabMAb

Anti-C5a-R 抗体 [EPR23278-117] - BSA and Azide free (ab272400)

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Flow Cytometry - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
  • Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23278-117] to C5a-R - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free
    C5a-R 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23278-117] to C5a-R - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human spleen and lung tissue. Clear cell carcinoma of human kidney tissue. ICC/IF: Human PBMCs. Flow Cyt: Human peripheral blood mononuclear cells.

  • 特記事項

    ab272400 is the carrier-free version of ab252435. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IP or WB.

    • ターゲット情報

    • 機能

      Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 翻訳後修飾

      Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ANAPHYLATOXIN RECEPTOR antibody
        • C5a anaphylatoxin chemotactic receptor antibody
        • C5a anaphylatoxin receptor antibody
        • C5A antibody
        • C5a ligand antibody
        • C5a-R antibody
        • C5aR antibody
        • C5AR_HUMAN antibody
        • c5ar1 antibody
        • C5R1 antibody
        • CD88 antibody
        • CD88 antigen antibody
        • Complement component 5 receptor 1 antibody
        • Complement component 5 receptor 2 antibody
        • Complement component 5a receptor 1 antibody
        see all

      画像

      • Flow Cytometry - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
        Flow Cytometry - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling C5a-R with ab252435 at 1/500 dilution (0.1µg)/ Right panel, compared with a Rabbit monoclonal IgG (ab172730) / Left panel, isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab252435 (Right). Then stained with anti-CD11b conjugated to BV421. Gated on viable cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

      • Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
        Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs labelling C5a-R with ab252435 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplamic and membranous staining in subsets of human PBMC. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 µg/ml dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

        Immunohistochemical analysis of paraffin-embedded clear cell carcinoma of human kidney tissue labeling C5a-R with ab252435 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on scattered cells in clear cell carcinoma of human kidney is observed. The section was incubated with ab252435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

        Immunohistochemical analysis of paraffin-embedded human lung tissue labeling C5a-R with ab252435 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on macrophages in human lung (PMID: 10027407) is observed. The section was incubated with ab252435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

        Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling C5a-R with ab252435 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic and membranous staining on mainly neutrophils in human spleen (PMID: 22691190) is observed. The section was incubated with ab252435 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252435).

      • Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)
        Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free (ab272400)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272400 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272400 は論文での使用が確認できていません。

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