Anti-C3 / C3b 抗体 [755] (ab11871)
Key features and details
- Mouse monoclonal [755] to C3 / C3b
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, Cynomolgus monkey
- Isotype: IgG2b
製品の概要
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製品名
Anti-C3 / C3b antibody [755]
C3 / C3b 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [755] to C3 / C3b -
由来種
Mouse -
特異性
This antibody reacts with C3 and C3b. See References multiple isoforms detected by WB. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human, Cynomolgus monkey -
免疫原
Full length native protein (purified) corresponding to Human C3/ C3b.
Database link: P01024 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
755 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 25254972
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IHC-P | (4) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 25254972 |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
組織特異性
Plasma. -
関連疾患
Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
翻訳後修飾
C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
- Unigene: 529053 Human
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別名
- Acylation-stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
see all
画像
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Immunohistochemistry analysis of Paraffin embedded section of human tonsil tissue sections labeling C3 / C3b with ab11871 at 1/200.
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ab11871 staining C3 / C3b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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Immunohistochemical analysis of Cynomolgus Monkey spleen tissue, staining C3 / C3b with ab11871.
Tissue was fixed with formaldehyde and blocked with blocking solution for 1 hour at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (1/50 in diluent) for 1 hour at 37°C. A biotinylated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab11871 は 23 報の論文で使用されています。
- Rumsey JW et al. Classical Complement Pathway Inhibition in a "Human-On-A-Chip" Model of Autoimmune Demyelinating Neuropathies. Adv Ther (Weinh) 5:N/A (2022). PubMed: 36211621
- Riba M et al. Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages. Cell Biosci 12:177 (2022). PubMed: 36307854
- Jia L et al. Single-cell profiling of infiltrating B cells and tertiary lymphoid structures in the TME of gastric adenocarcinomas. Oncoimmunology 10:1969767 (2021). PubMed: 34513317
- Smith VM et al. A Functional Human-on-a-Chip Autoimmune Disease Model of Myasthenia Gravis for Development of Therapeutics. Front Cell Dev Biol 9:745897 (2021). PubMed: 34881241
- Fan S et al. Extensive Sub-RPE Complement Deposition in a Nonhuman Primate Model of Early-Stage Diabetic Retinopathy. Invest Ophthalmol Vis Sci 62:30 (2021). PubMed: 33749721