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Recombinant fragment, corresponding to a region within amino acids 1498 - 1625 of Human C3.
Our Abpromise guarantee covers the use of ab97462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 187 kDa.|
|ICC/IF||1/100 - 1/1000.|
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling C3 with ab97462 at 1/200 dilution (green) in ICC/IF. Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes.
Nuclei were stained using Hoechst 33342 (blue).
Paraffin-embedded mouse brain tissue stained for C3 using ab97462 at 1/500 dilution in immunohistochemical analysis.
C3 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 μg of ab97462. Western blot was performed from the immunoprecipitate using ab97462.
Lane 1: HepG2 whole cell extract.
Lane 2: Control IgG instead of ab97462 in HepG2 whole cell extract.
Lane 3: ab97462 IP in HepG2 whole cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"