Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Adult brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton 0.3%
Specification
Adult brain
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS BSA 3% Triton 3%
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-rabbit 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
IF on frozen mouse sections fixed in PFA 4%. Nuclei were stained with DAPI. This antibody is good for A1 subtype of astrocytes.
Abcam response
Abcam has not tested ab11862 in IHC-FoFr and thus could not guarantee the antibody performance in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sébastien Brot
Verified customer
投稿 Jul 09 2019