Application
Western blot
Sample
Human Tissue lysate - other (Serum)
Loading amount
20 µg
Specification
Serum
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse HRP (GE Healthcare)
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140, 90, 62, 33 kDa
Additional data
Additional Notes
Plasma C3 is composed of several subunits in blood.
This antibody can detect some of subunits very clearly
This antibody can detect some of subunits very clearly
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
幸嗣 植田
Verified customer
投稿 Feb 16 2011