Anti-C2 抗体 [EPR17979] (ab209900)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17979] to C2
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-C2 antibody [EPR17979]
C2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17979] to C2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human serum, plasma and fetal kidney lysate; Mouse serum.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17979 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab209900の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
ターゲット情報
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機能
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
関連疾患
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 717 Human
- Entrez Gene: 12263 Mouse
- Omim: 217000 Human
- SwissProt: P06681 Human
- SwissProt: P21180 Mouse
- Unigene: 408903 Human
- Unigene: 283217 Mouse
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別名
- ARMD14 antibody
- C2 antibody
- C3/C5 convertase antibody
see all
画像
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All lanes : Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3: 3 minutes; Lane 2: 30 seconds.
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Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab209900 は 1 報の論文で使用されています。
- Pei J et al. Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak. BMC Vet Res 18:34 (2022). PubMed: 35031034